Tannia Vargas-Tierras, V. Morales-León, Sharon Andi-Barrera, Rubén Toapanta-Topón, María Morales-León, Lissette Segovia-Tello, Viviana Lara-Villegas
{"title":"通过验证电热原子吸收法检测水中的砷和铅离子","authors":"Tannia Vargas-Tierras, V. Morales-León, Sharon Andi-Barrera, Rubén Toapanta-Topón, María Morales-León, Lissette Segovia-Tello, Viviana Lara-Villegas","doi":"10.21931/rb/2024.09.01.8","DOIUrl":null,"url":null,"abstract":"The validation of the electrothermal spectrometry method for Arsenic and lead determination in water samples guarantees the quality of analytical data. The study was carried out at AQLAB. A theoretical and technical investigation was applied to ensure the reliability and accuracy of the analytical method. Parameters such as INEN standards, Eurachem international standards, AQLAB internal documents and environmental standards were used to validate the process. Several readings were performed on different samples to make calibration curves for As and Pb, evaluate the method's linearity, and obtain information on the slope, intercept and determination coefficient. Statistical calculations were used to determine the limit of detection and quantification, repeatability, reproducibility, trueness and uncertainty. The results show that the hypotheses were fulfilled, calibration curves with determination coefficient higher than 0.995, repeatability standard deviation lower than 16%, calculated F < critical F (4.96), calculated t student < theoretical (2.23), trueness was between 100.29-110.18 and 99.64-107.92%, uncertainty was 20% range 0.005 to 0.10 mg/L and 15% range 0.01-0.20mg/L, limit of detection was 0.001 and 0.005 mg/L and limit of quantification was 0.01 and 0.02mg/L, respectively. Therefore, the validation method was robust and accurate.\n \nKeywords: water samples, analytical data, reliable results, Arsenic, Lead, validation.","PeriodicalId":505112,"journal":{"name":"Bionatura","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Detection of arsenic and lead ions in water through validation of the electrothermal atomic absorption method\",\"authors\":\"Tannia Vargas-Tierras, V. Morales-León, Sharon Andi-Barrera, Rubén Toapanta-Topón, María Morales-León, Lissette Segovia-Tello, Viviana Lara-Villegas\",\"doi\":\"10.21931/rb/2024.09.01.8\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The validation of the electrothermal spectrometry method for Arsenic and lead determination in water samples guarantees the quality of analytical data. The study was carried out at AQLAB. A theoretical and technical investigation was applied to ensure the reliability and accuracy of the analytical method. Parameters such as INEN standards, Eurachem international standards, AQLAB internal documents and environmental standards were used to validate the process. Several readings were performed on different samples to make calibration curves for As and Pb, evaluate the method's linearity, and obtain information on the slope, intercept and determination coefficient. Statistical calculations were used to determine the limit of detection and quantification, repeatability, reproducibility, trueness and uncertainty. The results show that the hypotheses were fulfilled, calibration curves with determination coefficient higher than 0.995, repeatability standard deviation lower than 16%, calculated F < critical F (4.96), calculated t student < theoretical (2.23), trueness was between 100.29-110.18 and 99.64-107.92%, uncertainty was 20% range 0.005 to 0.10 mg/L and 15% range 0.01-0.20mg/L, limit of detection was 0.001 and 0.005 mg/L and limit of quantification was 0.01 and 0.02mg/L, respectively. Therefore, the validation method was robust and accurate.\\n \\nKeywords: water samples, analytical data, reliable results, Arsenic, Lead, validation.\",\"PeriodicalId\":505112,\"journal\":{\"name\":\"Bionatura\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-03-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Bionatura\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.21931/rb/2024.09.01.8\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bionatura","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.21931/rb/2024.09.01.8","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
对测定水样中砷和铅的电热光谱法进行验证可确保分析数据的质量。这项研究在 AQLAB 进行。为确保分析方法的可靠性和准确性,进行了理论和技术调查。在验证过程中使用了 INEN 标准、Eurachem 国际标准、AQLAB 内部文件和环境标准等参数。对不同的样品进行了多次读数,以绘制砷和铅的校准曲线,评估该方法的线性度,并获得斜率、截距和确定系数等信息。统计计算用于确定检测和定量限、重复性、再现性、真实度和不确定性。结果表明,假设成立,校准曲线的测定系数高于 0.995,重复性标准偏差低于 16%,计算 F < 临界 F(4.96),计算 t 学生 < 理论值(2.检出限分别为 0.001 和 0.005 mg/L,定量限分别为 0.01 和 0.02 mg/L。因此,该方法可靠、准确。关键词:水样;分析数据;结果可靠;砷;铅;验证。
Detection of arsenic and lead ions in water through validation of the electrothermal atomic absorption method
The validation of the electrothermal spectrometry method for Arsenic and lead determination in water samples guarantees the quality of analytical data. The study was carried out at AQLAB. A theoretical and technical investigation was applied to ensure the reliability and accuracy of the analytical method. Parameters such as INEN standards, Eurachem international standards, AQLAB internal documents and environmental standards were used to validate the process. Several readings were performed on different samples to make calibration curves for As and Pb, evaluate the method's linearity, and obtain information on the slope, intercept and determination coefficient. Statistical calculations were used to determine the limit of detection and quantification, repeatability, reproducibility, trueness and uncertainty. The results show that the hypotheses were fulfilled, calibration curves with determination coefficient higher than 0.995, repeatability standard deviation lower than 16%, calculated F < critical F (4.96), calculated t student < theoretical (2.23), trueness was between 100.29-110.18 and 99.64-107.92%, uncertainty was 20% range 0.005 to 0.10 mg/L and 15% range 0.01-0.20mg/L, limit of detection was 0.001 and 0.005 mg/L and limit of quantification was 0.01 and 0.02mg/L, respectively. Therefore, the validation method was robust and accurate.
Keywords: water samples, analytical data, reliable results, Arsenic, Lead, validation.