CircITGA7 通过靶向 miR-330-3p/KLF10 轴调节膀胱癌细胞的恶性表型。

The Kaohsiung journal of medical sciences Pub Date : 2024-04-01 Epub Date: 2024-03-25 DOI:10.1002/kjm2.12821
Xian-Xu Yang, Chao Wang
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引用次数: 0

摘要

膀胱癌(BCa)是常见的恶性肿瘤之一。环状 RNA(circRNA)在癌症进展中起着调控作用。CircITGA7 是由 ITGA7 的几个外显子产生的 circRNA。circITGA7 在 BCa 中的潜在作用仍然未知,有待探索。研究采用定量实时聚合酶链反应(qRT-PCR)评估 circITGA7 和 miR-330-3p 在 BCa 组织和细胞系中的表达。Kaplan-Meier 分析用于评估这些 BCa 患者的总生存率。利用CCK-8、EdU、伤口愈合和Transwell试验,通过过表达circITGA7检测circITGA7的生物功能。为了进一步验证体外实验结果,还进行了异种移植实验。为了探索circITGA7的作用机制,研究人员采用了荧光素酶报告、RNA牵引、荧光原位杂交(FISH)等方法来检测circITGA7、miR-330-3p和克鲁珀尔样因子10(KLF10)之间的结合相互作用。在 BCa 组织和细胞系中,circITGA7 被下调,表明总存活期延长。此外,circITGA7通过负调控miR-330-3p限制了BCa的细胞增殖、迁移和侵袭。体内模型显示,circITGA7影响了肿瘤的生长。此外,miR-330-3p 的过表达通过直接靶向 KLF10 促进了细胞的进展。CircITGA7通过circITGA7/hsa-miR-330-3p/KLF10轴缓解了BCa细胞的进展,这可能为BCa提供了新的治疗靶点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
CircITGA7 regulates malignant phenotypes in bladder cancer cells via targeting miR-330-3p/KLF10 axis.

Bladder cancer (BCa) is one of the common malignancies. Circular RNAs (circRNAs) play regulatory roles in cancer progression. CircITGA7 is a circRNA generated from several exons of ITGA7. The potential role of circITGA7 in BCa remains unknown and needs to be explored. Quantitative real time polymerase chain reaction (qRT-PCR) was used to assess circITGA7 and miR-330-3p expression in BCa tissues and cell lines. Kaplan-Meier analysis was used to evaluate the overall survival of these BCa patients. The biological function of circITGA7 was examined by overexpression of circITGA7 using CCK-8, EdU, wound-healing, and Transwell assays. Xenograft assay was performed to further validate the in vitro results. To explore the mechanism of circITGA7, luciferase reporter, RNA pull-down, fluorescence in situ hybridization (FISH) assays were employed to examine the binding interaction among circITGA7, miR-330-3p and kruppel-like factor 10 (KLF10). Western blot was used to study the protein levels of KLF10.CircITGA7 was downregulated in BCa tissues and cell lines and indicated longer overall survival. Moreover, circITGA7 restricted cell proliferation, migration and invasion of BCa through negatively regulating miR-330-3p. The in vivo model showed that circITGA7 influenced the tumor growth. Besides, the overexpression of miR-330-3p promoted cell progression by directly targeting KLF10. Mechanistically, circITGA7 inhibited BCa progression by activating KLF10 via targeting miR-330-3p.CircITGA7 alleviates BCa cell progression via circITGA7/hsa-miR-330-3p/KLF10 axis, which may provide novel therapeutic targets for BCa.

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