用冷冻电子显微镜和分子建模方法描述与微管结合的 Tau 的动力学特征

Q4 Biochemistry, Genetics and Molecular Biology

Methods in molecular biology Pub Date : 2024-01-01 DOI:10.1007/978-1-0716-3629-9_4

Z Faidon Brotzakis

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引用次数: 0

摘要

电子显微镜元推理综合结构生物学方法能够将冷冻电子显微镜电子密度图与分子动力学等分子建模技术相结合，以高效的方式揭示原子论生物分子结构集合和图谱数据的误差。在此，我们展示了电子显微镜元参考方案和分析方法，通过使用最先进的分子力学力场和电子密度图，阐明了与微管结合的微管相关蛋白tau的原子结构组合。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Cryo-electron Microscopy and Molecular Modeling Methods to Characterize the Dynamics of Tau Bound to Microtubules.

The electron microscopy metainference integrative structural biology method enables the combination of cryo-electron microscopy electron density maps with molecular modeling techniques such as molecular dynamics to unveil the atomistic biomolecular structural ensemble and the error in the map data in an efficient manner. Here we illustrate the electron microscopy metainference protocol and analysis used to elucidate the atomistic structural ensemble of the microtubule-associated protein tau bound to microtubules by using state-of-the-art molecular mechanic force field and the electron density map.

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来源期刊

Methods in molecular biology Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

2.00

自引率

0.00%

发文量

3536

期刊介绍： For over 20 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by-step fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice.