在猪模型中鉴定下颌骨缺损损伤和治疗手术后骨质再生过程中口腔细菌的时间转移。

IF 2.8 3区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE
Molecular Oral Microbiology Pub Date : 2024-10-01 Epub Date: 2024-03-21 DOI:10.1111/omi.12460
Fatemeh Sanjar, David T Silliman, Ian J Johnson, Zayer Htut, Trent J Peacock, Samira F Thompson, Gregory R Dion, Md A Nahid, John F Decker, Kai P Leung
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引用次数: 0

摘要

背景:人类口腔生态系统被认为是仅次于肠道的第二大、最多样化的微生物群,其微生物种类繁多且具有特异性。尽管越来越多的证据表明口腔微生物群在支持健康的免疫系统和预防局部及全身感染方面的重要性,但颅颌面(CMF)创伤和常规整形手术治疗对口腔常驻微生物群落结构和功能的影响仍然未知。颅颌面外伤影响到大量人群,需要进行广泛的康复治疗,造成长期的发病率和生产力损失。由于宿主免疫功能减弱以及口腔微生物群落中菌群失调导致的定植抵抗力降低,口腔生态系统中机会性共生菌或耐多药病原体的过度生长会使治疗效果变得复杂。目的:为了了解在 CMF 损伤和后续治疗过程中微生物群落结构的动态变化,我们诱导汉福德小型猪(n = 3)牙槽上下颌骨缺损,并比较了即刻下颌骨重建(IMR)和延迟下颌骨重建(DMR)手术的治疗方法:采用细菌 16S 核糖体 RNA 基因标记测序法,调查了未受伤上颌骨(对照组)和接受 DMR 治疗的受伤左下颌骨(舌侧和颊侧)在伤口愈合后 70 天内的细菌群落组成和丰度。对于接受 IMR 治疗的受伤右下颌骨,微生物组成和丰度的调查一直持续到伤口愈合后 14 天。此外,我们还测量了血清中与骨再生和愈合相关的生化标志物(如骨钙素)的水平。我们使用计算机断层扫描测量并比较了接受DMR和IMR治疗方法的部位的下颌骨特征,如小梁厚度,直到第140天,即研究期结束:无论采用 IMR 还是 DMR 治疗方法,我们都观察到在 CMF 损伤后和/或治疗后,粘膜细菌居民发生了类似的菌群失调和转移。在规定的愈合天数内,镰刀菌科、卟啉菌科和类杆菌科细菌大量繁殖,而巴斯德氏菌科、摩拉氏菌科和奈瑟氏菌科细菌相对丰富度下降。我们还观察到,在第 0 天和第 56 天,由于时间不稳定性和动物间异质性的降低,物种丰富度和丰度也有所下降,第 0 天对应 DMR 组的损伤,第 56 天对应 DMR 组的延迟治疗或 IMR 组的损伤和即时治疗。对骨愈合特征的分析表明,IMR 与 DMR 治疗方法的骨愈合情况相当。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Identification of temporal shifts of oral bacteria in bone regeneration following mandibular bone defect injury and therapeutic surgery in a porcine model.

Background: Considered the second largest and most diverse microbiome after the gut, the human oral ecosystem is complex with diverse and niche-specific microorganisms. Although evidence is growing for the importance of oral microbiome in supporting a healthy immune system and preventing local and systemic infections, the influence of craniomaxillofacial (CMF) trauma and routine reconstructive surgical treatments on community structure and function of oral resident microbes remains unknown. CMF injuries affect a large number of people, needing extensive rehabilitation with lasting morbidity and loss of human productivity. Treatment efficacy can be complicated by the overgrowth of opportunistic commensals or multidrug-resistant pathogens in the oral ecosystem due to weakened host immune function and reduced colonization resistance in a dysbiotic oral microbiome.

Aims: To understand the dynamics of microbiota's community structure during CMF injury and subsequent treatments, we induced supra-alveolar mandibular defect in Hanford miniature swine (n = 3) and compared therapeutic approaches of immediate mandibullar reconstructive (IMR) versus delayed mandibullar reconstructive (DMR) surgeries.

Methods: Using bacterial 16S ribosomal RNA gene marker sequencing, the composition and abundance of the bacterial community of the uninjured maxilla (control) and the injured left mandibula (lingual and buccal) treated by DMR were surveyed up to 70-day post-wounding. For the injured right mandibula receiving IMR treatment, the microbial composition and abundance were surveyed up to 14-day post-wounding. Moreover, we measured sera level of biochemical markers (e.g., osteocalcin) associated with bone regeneration and healing. Computed tomography was used to measure and compare mandibular bone characteristics such as trabecular thickness between sites receiving DMR and IMR therapeutic approaches until day 140, the end of study period.

Results: Independent of IMR versus DMR therapy, we observed similar dysbiosis and shifts of the mucosal bacteria residents after CMF injury and/or following treatment. There was an enrichment of Fusobacterium, Porphyromonadaceae, and Bacteroidales accompanied by a decline in Pasteurellaceae, Moraxella, and Neisseria relative abundance in days allotted for healing. We also observed a decline in species richness and abundance driven by reduction in temporal instability and inter-animal heterogeneity on days 0 and 56, with day 0 corresponding to injury in DMR group and day 56 corresponding to delayed treatment for DMR or injury and immediate treatment for the IMR group. Analysis of bone healing features showed comparable bone-healing profiles for IMR vs. DMR therapeutic approach.

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来源期刊
Molecular Oral Microbiology
Molecular Oral Microbiology DENTISTRY, ORAL SURGERY & MEDICINE-MICROBIOLOGY
CiteScore
6.50
自引率
5.40%
发文量
46
审稿时长
>12 weeks
期刊介绍: Molecular Oral Microbiology publishes high quality research papers and reviews on fundamental or applied molecular studies of microorganisms of the oral cavity and respiratory tract, host-microbe interactions, cellular microbiology, molecular ecology, and immunological studies of oral and respiratory tract infections. Papers describing work in virology, or in immunology unrelated to microbial colonization or infection, will not be acceptable. Studies of the prevalence of organisms or of antimicrobials agents also are not within the scope of the journal. The journal does not publish Short Communications or Letters to the Editor. Molecular Oral Microbiology is published bimonthly.
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