雌性小鼠血清外泌体微RNA谱的动态变化:化学诱导的雌性绝经和雌激素治疗的挽救。

IF 5.3 2区 医学 Q1 GERIATRICS & GERONTOLOGY
GeroScience Pub Date : 2024-12-01 Epub Date: 2024-03-19 DOI:10.1007/s11357-024-01129-9
Bianka Machado Zanini, Bianca Machado de Avila, Driele Neske Garcia, Jéssica Damé Hense, Gabriel Barreto Veiga, Mariana Machado Barreto, Sarah Ashiqueali, Jeffrey B Mason, Hariom Yadav, Michal Masternak, Augusto Schneider
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引用次数: 0

摘要

卵巢储备功能下降导致绝经和血清雌激素减少。微RNA是一种小型非编码RNA,可调节基因表达,由细胞分泌,并通过外泌体进入血清。血清中的 miRNA 可调控组织功能和疾病发展。因此,本研究旨在鉴定雌激素诱导并用 17β-estradiol (E2) 治疗的小鼠血清外泌体中的 miRNA 图谱。雌性小鼠分为三组,包括对照组(CTL)、注射 4-乙烯基环己烯二环氧化物(VCD)组和注射 VCD 加 E2 组(VCD + E2)。无周期性和卵泡数量的显著减少证实了雌激素减少(p
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Dynamics of serum exosome microRNA profile altered by chemically induced estropause and rescued by estrogen therapy in female mice.

Dynamics of serum exosome microRNA profile altered by chemically induced estropause and rescued by estrogen therapy in female mice.

The decline in the ovarian reserve leads to menopause and reduced serum estrogens. MicroRNAs are small non-coding RNAs, which can regulate gene expression and be secreted by cells and trafficked in serum via exosomes. Serum miRNAs regulate tissue function and disease development. Therefore, the aim of this study was to identify miRNA profiles in serum exosomes of mice induced to estropause and treated with 17β-estradiol (E2). Female mice were divided into three groups including control (CTL), injected with 4-Vinylcyclohexene diepoxide (VCD), and injected with VCD plus E2 (VCD + E2). Estropause was confirmed by acyclicity and a significant reduction in the number of ovarian follicles (p < 0.05). Body mass gain during estropause was higher in VCD and VCD + E2 compared to CTL females (p = 0.02). Sequencing of miRNAs was performed from exosomes extracted from serum, and 402 miRNAs were detected. Eight miRNAs were differentially regulated between CTL and VCD groups, seven miRNAs regulated between CTL and VCD + E2 groups, and ten miRNAs regulated between VCD and VCD + E2 groups. Only miR-200a-3p and miR-200b-3p were up-regulated in both serum exosomes and ovarian tissue in both VCD groups, suggesting that these exosomal miRNAs could be associated with ovarian activity. In the hepatic tissue, only miR-370-3p (p = 0.02) was up-regulated in the VCD + E2 group, as observed in serum. Our results suggest that VCD-induced estropause and E2 replacement have an impact on the profile of serum exosomal miRNAs. The miR-200 family was increased in serum exosomes and ovarian tissue and may be a candidate biomarker of ovarian function.

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来源期刊
GeroScience
GeroScience Medicine-Complementary and Alternative Medicine
CiteScore
10.50
自引率
5.40%
发文量
182
期刊介绍: GeroScience is a bi-monthly, international, peer-reviewed journal that publishes articles related to research in the biology of aging and research on biomedical applications that impact aging. The scope of articles to be considered include evolutionary biology, biophysics, genetics, genomics, proteomics, molecular biology, cell biology, biochemistry, endocrinology, immunology, physiology, pharmacology, neuroscience, and psychology.
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