利用 MALDI-TOF/MS 通过 TAGs 图谱分析鉴定大豆产地

IF 2.6 3区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY
Guangfeng Zeng, Zhiyuan Wang, Yingye Hou, Bo Ding, Lu Wang, Wenrui Chen, Ju Li, Jianjun Xie
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引用次数: 0

摘要

大豆具有氨基酸种类均衡、营养价值高的特点,是世界上主要的油料作物。为了研究三酰甘油(TAGs)在追溯中国进口大豆地理来源方面的潜力,采用基质辅助激光解吸电离质谱法(MALDI-MS)对大豆油中的 TAGs 进行了分析。根据获得的 MALDI-MS 图谱,应用正交偏最小二乘判别分析(OPLS-DA)建立识别模型,以追溯四个典型产地(阿根廷、美国、巴西和加拿大)的大豆来源。通过 40 个测试集样本对模型进行了验证,OPLS-DA 模型的综合识别准确率达到 100%。本研究的方法和模型准确可靠,能准确识别大豆的地理产地。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Identification of Soybean Origin via TAGs Profile Analysis Using MALDI-TOF/MS

Identification of Soybean Origin via TAGs Profile Analysis Using MALDI-TOF/MS

Soybeans have the characteristics of balanced amino acid species and high nutritional value and served as the main oil crop in the world. In order to investigate the potential of triacylglycerols (TAGs) for tracing geographic origin of imported soybeans in China, matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) was used to profile TAGs in soybean oils. Orthogonal partial least-squares discrimination analysis(OPLS-DA) was applied to establish identification model based on the acquired MALDI-MS spectra to trace the soybean origin of four typical origins (Argentina, the USA, Brazil, and Canada). The models were verified through 40 samples of the test set, and the comprehensive identification accuracy rate of the OPLS-DA models reached 100%. The method and model in this study were accurate and reliable, and could accurately identify the geographic origin of soybean.

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来源期刊
Food Analytical Methods
Food Analytical Methods 农林科学-食品科技
CiteScore
6.00
自引率
3.40%
发文量
244
审稿时长
3.1 months
期刊介绍: Food Analytical Methods publishes original articles, review articles, and notes on novel and/or state-of-the-art analytical methods or issues to be solved, as well as significant improvements or interesting applications to existing methods. These include analytical technology and methodology for food microbial contaminants, food chemistry and toxicology, food quality, food authenticity and food traceability. The journal covers fundamental and specific aspects of the development, optimization, and practical implementation in routine laboratories, and validation of food analytical methods for the monitoring of food safety and quality.
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