George Sirinakis, Edward S. Allgeyer, Dmitry Nashchekin, and Daniel St. Johnston
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引用次数: 0
摘要
在这项工作中,我们提出了一种斜平面显微镜,其设计可与市售显微镜底座无缝配合。为了支持显微镜底座提供的所有功能(其中物镜的位置并不固定),我们采用了双镜扫描几何结构,可以在显微镜常规操作过程中补偿物镜位置的变化。我们的研究表明,在 100 倍、1.35 NA 物镜偏离设计位置 ± 1 mm 的位移范围内,PSF 尺寸在横向和轴向分别增加了 3% 和 11%,而在焦平面周围 ± 10 µm 的范围内,放大率误差为 0.5%。与更传统的扫描镜头/巨像镜组合相比,双镜扫描几何具有更高的光效和更紧凑的占地面积,这对所有 OPM 设计都有好处,无论是否使用商用底座。
User-friendly oblique plane microscopy on a fully functional commercially available microscope base
In this work we present an oblique plane microscope designed to work seamlessly with a commercially available microscope base. To support all the functionality offered by the microscope base, where the position of the objective lens is not fixed, we adopted a two-mirror scanning geometry that can compensate for changes to the position of the objective lens during routine microscope operation. We showed that within a ± 1 mm displacement range of the 100X, 1.35 NA objective lens away from its designed position, the PSF size increased by <3% and <11% in the lateral and axial dimensions, respectively, while the error in magnification was <0.5% within volumes extending ± 10 µm about the focal plane. Compared to the more traditional scan-lens/galvo-mirror combination, the two-mirror scanning geometry offers higher light efficiency and a more compact footprint, which could be beneficial to all OPM designs regardless of the use of a commercial base or not.
期刊介绍:
The journal''s scope encompasses fundamental research, technology development, biomedical studies and clinical applications. BOEx focuses on the leading edge topics in the field, including:
Tissue optics and spectroscopy
Novel microscopies
Optical coherence tomography
Diffuse and fluorescence tomography
Photoacoustic and multimodal imaging
Molecular imaging and therapies
Nanophotonic biosensing
Optical biophysics/photobiology
Microfluidic optical devices
Vision research.