人类支气管上皮细胞转录组对不同炎症状态患者血清的反应变化

IF 4.6 2区 医学 Q1 RESPIRATORY SYSTEM
Lung Pub Date : 2024-04-01 Epub Date: 2024-03-17 DOI:10.1007/s00408-024-00679-1
Kokilavani Sivaraman, Bin Liu, Beatriz Martinez-Delgado, Julia Held, Manuela Büttner, Thomas Illig, Sonja Volland, Gema Gomez-Mariano, Nils Jedicke, Tetyana Yevsa, Tobias Welte, David S DeLuca, Sabine Wrenger, Beata Olejnicka, Sabina Janciauskiene
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引用次数: 0

摘要

目的:研究人类支气管上皮细胞(HBEC)对不同炎症程度患者血清反应的转录组:方法:使用从汉诺威统一生物库(Hannover Unified Biobank)获得的 19 名 COVID-19 患者的血清。采样时,5 名患者的 WHO 临床进展量表(WHO-CPS)评分为 9(重症)。其余 14 名患者的 WHO-CPS 评分低于 9(范围为 1-7),病情较轻。采用多重免疫测定法评估血清炎症指标。在 HBEC 培养液中添加 2% 的患者血清,在 37 °C、5% CO2 条件下培养细胞 18 小时,然后将细胞 RNA 用于 RNA-Seq 分析:基于 500 个 RNA-Seq "核心基因 "的主成分分析将细胞分为两个子集:暴露于血清的 4 个子集(I)和 15 个子集(II)。与用 14 名患者血清(得分在 1-7 之间,其中一名患者得分 = 9)治疗的子集 II 细胞相比,用 4 名患者血清(得分 9)治疗的子集 I 细胞有 5566 个差异表达基因,其中 2793 个基因上调,2773 个基因下调。在子集 I 细胞中,观察到 TLR4 和 CXCL8 的表达较高,但 CDH1、ACE2 和 HMOX1 的表达较低,参与代谢调节、细胞骨架组织和激酶活性途径的基因受到的影响较大:这种简单的模型可用于描述患者血清和上皮细胞的特性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Human Bronchial Epithelial Cell Transcriptome Changes in Response to Serum from Patients with Different Status of Inflammation.

Human Bronchial Epithelial Cell Transcriptome Changes in Response to Serum from Patients with Different Status of Inflammation.

Purpose: To investigate the transcriptome of human bronchial epithelial cells (HBEC) in response to serum from patients with different degrees of inflammation.

Methods: Serum from 19 COVID-19 patients obtained from the Hannover Unified Biobank was used. At the time of sampling, 5 patients had a WHO Clinical Progression Scale (WHO-CPS) score of 9 (severe illness). The remaining 14 patients had a WHO-CPS of below 9 (range 1-7), and lower illness. Multiplex immunoassay was used to assess serum inflammatory markers. The culture medium of HBEC was supplemented with 2% of the patient's serum, and the cells were cultured at 37 °C, 5% CO2 for 18 h. Subsequently, cellular RNA was used for RNA-Seq.

Results: Patients with scores below 9 had significantly lower albumin and serum levels of E-selectin, IL-8, and MCP-1 than patients with scores of 9. Principal component analysis based on 500 "core genes" of RNA-seq segregated cells into two subsets: exposed to serum from 4 (I) and 15 (II) patients. Cells from a subset (I) treated with serum from 4 patients with a score of 9 showed 5566 differentially expressed genes of which 2793 were up- and 2773 downregulated in comparison with cells of subset II treated with serum from 14 patients with scores between 1 and 7 and one with score = 9. In subset I cells, a higher expression of TLR4 and CXCL8 but a lower CDH1, ACE2, and HMOX1, and greater effects on genes involved in metabolic regulation, cytoskeletal organization, and kinase activity pathways were observed.

Conclusion: This simple model could be useful to characterize patient serum and epithelial cell properties.

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来源期刊
Lung
Lung 医学-呼吸系统
CiteScore
9.10
自引率
10.00%
发文量
95
审稿时长
6-12 weeks
期刊介绍: Lung publishes original articles, reviews and editorials on all aspects of the healthy and diseased lungs, of the airways, and of breathing. Epidemiological, clinical, pathophysiological, biochemical, and pharmacological studies fall within the scope of the journal. Case reports, short communications and technical notes can be accepted if they are of particular interest.
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