利用单细胞方法研究植物维管发育

IF 8.3 2区 生物学 Q1 PLANT SCIENCES
Claudia von der Mark , Max Minne , Bert De Rybel
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引用次数: 0

摘要

血管细胞是一种高度复杂的异质组织。其组成、功能、形状和排列随发育阶段、器官和物种的不同而变化。因此,要了解支撑这种复杂性的转录调控,需要一种能够捕捉血管细胞形成过程中快速事件的高分辨率技术。单细胞和单核 RNA 测序(sc/snRNA-seq)方法提供了强大的工具,可从这些含量低且动态变化的细胞类型中提取转录信息,从而重建发育轨迹。在此,我们总结并反思了最近利用单细胞转录组学研究血管细胞类型的研究,并讨论了目前和未来在血管发育领域实施 sc/snRNA-seq 方法的情况。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Studying plant vascular development using single-cell approaches

Vascular cells form a highly complex and heterogeneous tissue. Its composition, function, shape, and arrangement vary with the developmental stage and between organs and species. Understanding the transcriptional regulation underpinning this complexity thus requires a high-resolution technique that is capable of capturing rapid events during vascular cell formation. Single-cell and single-nucleus RNA sequencing (sc/snRNA-seq) approaches provide powerful tools to extract transcriptional information from these lowly abundant and dynamically changing cell types, which allows the reconstruction of developmental trajectories. Here, we summarize and reflect on recent studies using single-cell transcriptomics to study vascular cell types and discuss current and future implementations of sc/snRNA-seq approaches in the field of vascular development.

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来源期刊
Current opinion in plant biology
Current opinion in plant biology 生物-植物科学
CiteScore
16.30
自引率
3.20%
发文量
131
审稿时长
6-12 weeks
期刊介绍: Current Opinion in Plant Biology builds on Elsevier's reputation for excellence in scientific publishing and long-standing commitment to communicating high quality reproducible research. It is part of the Current Opinion and Research (CO+RE) suite of journals. All CO+RE journals leverage the Current Opinion legacy - of editorial excellence, high-impact, and global reach - to ensure they are a widely read resource that is integral to scientists' workflow.
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