闭锁卵泡中高表达的 circRALGPS2 通过编码一种新蛋白诱导鸡颗粒细胞凋亡和自噬

IF 7 1区农林科学 Q1 Agricultural and Biological Sciences

Journal of Animal Science and Biotechnology Pub Date : 2024-03-11 DOI:10.1186/s40104-024-01003-w

Haorong He, Yuanhang Wei, Yuqi Chen, Xiyu Zhao, Xiaoxu Shen, Qing Zhu, Huadong Yin

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引用次数: 0

摘要

鸡的繁殖性能主要取决于卵泡的发育。卵泡发育异常会导致鸡的繁殖性能下降，甚至引发卵巢疾病。鸡是自发性卵巢癌高发的唯一非人类动物。近年来，circRNAs参与卵泡发育和闭锁调节的研究已得到证实。在本研究中，我们利用健康和闭锁的鸡卵泡进行了circRNA RNC-seq分析。结果显示，circRALGPS2的表达存在差异。随后我们证实，circRALGPS2可转化为具有IRES活性的蛋白质，命名为circRALGPS2-212aa。接着，我们发现circRALGPS2-212aa通过与PARP1和HMGB1形成复合物，促进鸡颗粒细胞的凋亡和自噬。我们的研究结果表明，circRALGPS2可通过circRALGPS2-212aa/PARP1/HMGB1轴调控鸡颗粒细胞的凋亡和自噬。

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High expression circRALGPS2 in atretic follicle induces chicken granulosa cell apoptosis and autophagy via encoding a new protein

The reproductive performance of chickens mainly depends on the development of follicles. Abnormal follicle development can lead to decreased reproductive performance and even ovarian disease among chickens. Chicken is the only non-human animal with a high incidence of spontaneous ovarian cancer. In recent years, the involvement of circRNAs in follicle development and atresia regulation has been confirmed. In the present study, we used healthy and atretic chicken follicles for circRNA RNC-seq. The results showed differential expression of circRALGPS2. It was then confirmed that circRALGPS2 can translate into a protein, named circRALGPS2-212aa, which has IRES activity. Next, we found that circRALGPS2-212aa promotes apoptosis and autophagy in chicken granulosa cells by forming a complex with PARP1 and HMGB1. Our results revealed that circRALGPS2 can regulate chicken granulosa cell apoptosis and autophagy through the circRALGPS2-212aa/PARP1/HMGB1 axis.

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来源期刊

Journal of Animal Science and Biotechnology AGRICULTURE, DAIRY & ANIMAL SCIENCE-

CiteScore

9.90

自引率

2.90%

发文量

822

审稿时长

17 weeks

期刊介绍： Journal of Animal Science and Biotechnology is an open access, peer-reviewed journal that encompasses all aspects of animal science and biotechnology. That includes domestic animal production, animal genetics and breeding, animal reproduction and physiology, animal nutrition and biochemistry, feed processing technology and bioevaluation, animal biotechnology, and meat science.