{"title":"犬口腔乳头状瘤病的细胞增殖和凋亡。","authors":"Emin Karakurt, Nuvit Coskun, Ugur Aydin, Serpil Dag, Enver Beytut, Veysel Soydal Ataseven, Volkan Yilmaz, Firat Dogan, Hilmi Nuhoglu, Celal Sahin Ermutlu, Ayfer Yildiz","doi":"10.30466/vrf.2023.1996086.3818","DOIUrl":null,"url":null,"abstract":"<p><p>This study was aimed at the evaluation of cell proliferation, <i>p53</i> level and apoptotic index by immunohistochemical methods in canine oral papillomatosis. The study material comprised of tumor tissue samples taken from six dogs being admitted to the Pathology Department of Faculty of Veterinary Medicine, Kafkas University, Kars, Türkiye. Choice of immunohistochemical staining was avidin-biotin peroxidase method. Cases of canine oral papillomatosis, determined to have been caused by canine papillomavirus-1, were found to have a rather high cell proliferation index. Furthermore, all cases were immunohisto-chemically demonstrated to carry a mutant <i>p53</i> gene. Despite the mutation of <i>p53</i> gene, the shift in the <i>Bax</i>/<i>Bcl-2</i> ratio of dogs diagnosed with tumor was in favor of the pro-apoptotic <i>Bax</i> gene. The apoptotic mechanism was determined to occur through both the caspase-dependent and caspase-independent pathways. While the lesions occupied the entire oral cavity in some cases, histopathologically, malignant transformation was not detected in any of the six cases.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":null,"pages":null},"PeriodicalIF":0.8000,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10924297/pdf/","citationCount":"0","resultStr":"{\"title\":\"Cell proliferation and apoptosis in canine oral papillomatosis.\",\"authors\":\"Emin Karakurt, Nuvit Coskun, Ugur Aydin, Serpil Dag, Enver Beytut, Veysel Soydal Ataseven, Volkan Yilmaz, Firat Dogan, Hilmi Nuhoglu, Celal Sahin Ermutlu, Ayfer Yildiz\",\"doi\":\"10.30466/vrf.2023.1996086.3818\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>This study was aimed at the evaluation of cell proliferation, <i>p53</i> level and apoptotic index by immunohistochemical methods in canine oral papillomatosis. The study material comprised of tumor tissue samples taken from six dogs being admitted to the Pathology Department of Faculty of Veterinary Medicine, Kafkas University, Kars, Türkiye. Choice of immunohistochemical staining was avidin-biotin peroxidase method. Cases of canine oral papillomatosis, determined to have been caused by canine papillomavirus-1, were found to have a rather high cell proliferation index. Furthermore, all cases were immunohisto-chemically demonstrated to carry a mutant <i>p53</i> gene. Despite the mutation of <i>p53</i> gene, the shift in the <i>Bax</i>/<i>Bcl-2</i> ratio of dogs diagnosed with tumor was in favor of the pro-apoptotic <i>Bax</i> gene. The apoptotic mechanism was determined to occur through both the caspase-dependent and caspase-independent pathways. While the lesions occupied the entire oral cavity in some cases, histopathologically, malignant transformation was not detected in any of the six cases.</p>\",\"PeriodicalId\":23989,\"journal\":{\"name\":\"Veterinary Research Forum\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.8000,\"publicationDate\":\"2024-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10924297/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Veterinary Research Forum\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.30466/vrf.2023.1996086.3818\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/2/15 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q3\",\"JCRName\":\"ZOOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Veterinary Research Forum","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.30466/vrf.2023.1996086.3818","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/2/15 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"ZOOLOGY","Score":null,"Total":0}
Cell proliferation and apoptosis in canine oral papillomatosis.
This study was aimed at the evaluation of cell proliferation, p53 level and apoptotic index by immunohistochemical methods in canine oral papillomatosis. The study material comprised of tumor tissue samples taken from six dogs being admitted to the Pathology Department of Faculty of Veterinary Medicine, Kafkas University, Kars, Türkiye. Choice of immunohistochemical staining was avidin-biotin peroxidase method. Cases of canine oral papillomatosis, determined to have been caused by canine papillomavirus-1, were found to have a rather high cell proliferation index. Furthermore, all cases were immunohisto-chemically demonstrated to carry a mutant p53 gene. Despite the mutation of p53 gene, the shift in the Bax/Bcl-2 ratio of dogs diagnosed with tumor was in favor of the pro-apoptotic Bax gene. The apoptotic mechanism was determined to occur through both the caspase-dependent and caspase-independent pathways. While the lesions occupied the entire oral cavity in some cases, histopathologically, malignant transformation was not detected in any of the six cases.
期刊介绍:
Veterinary Research Forum (VRF) is a quarterly international journal committed to publish worldwide contributions on all aspects of veterinary science and medicine, including anatomy and histology, physiology and pharmacology, anatomic and clinical pathology, parasitology, microbiology, immunology and epidemiology, food hygiene, poultry science, fish and aquaculture, anesthesia and surgery, large and small animal internal medicine, large and small animal reproduction, biotechnology and diagnostic imaging of domestic, companion and farm animals.