鉴定具有抗菌潜能的刺五加叶内生真菌

Gusti Ayu Widayanti, H. Widjajanti, S. Salni, A. Sutjipto
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摘要

内生真菌是生活在植物组织中的微生物,能合成与其宿主相同的活性生化化合物。本研究旨在确定Jeruju叶(Acanthus ilicifolius)分离物DJS1的内生真菌类型。本研究于 2020 年 1 月进行,属于描述性定性研究。研究对象是一种具有抗菌潜力的内生真菌分离物。通过观察宏观和微观形态表型特征,鉴定分离出的具有抗菌潜力的Jeruju叶(Acanthus ilicifolius)内生真菌DJS1。宏观观察描述了真菌分离物上出现的菌落结构形式、气生菌丝形式和放射线。显微镜观察是为了观察菌丝的形状和分生孢子的形状。分子观察是通过使用 ITS 引物扩增 DNA 进行的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Identification of Endophytic Fungi on the Leaves of Jeruju (Acanthus ilicifolius) which are Potential as Antibacteria
Endophytic fungi are microbes that live in plant tissues and can synthesize the same active biochemical compounds as their hosts. This study aims to determine the type of endophytic fungi of Jeruju leaves (Acanthus ilicifolius) isolate DJS1. This research was conducted in January 2020 and is a type of descriptive qualitative research. The subjects in this study were one type of endophytic fungi isolate that has the potential as an antibacterial. Identification of endophytic fungi of Jeruju leaves (Acanthus ilicifolius) isolated DJS1 which have antibacterial potential by observing macroscopic and microscopic morphological phenotypic characters. Macroscopic observations described the form of colony structure, aerial hyphae forms, and radial lines that appeared on the fungal isolates. Microscopic observations were made to observe the shape of the hyphae and the shape of the conidia. While molecular observations were carried out by amplifying DNA using ITS primers.
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