Z. Kmieć, J. Kieżun, B. Krazinski, Przemysław Kwiatkowski, J. Godlewski
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引用次数: 0
摘要
本文概述了对结直肠癌(CRC)组织和非受累结肠壁中伽蓝宁(Gal)(肠神经系统中含量丰富的神经肽之一)及其三种受体(GalR1-3)的免疫组化(IHC)和生化研究,以及它们与 CRC 患者临床病理数据的关联。我们首次从形态学角度证明了 CRC 切片中内源性 Gal 的存在,并测量了肿瘤组织匀浆和未受影响的结肠壁切片中 Gal 的含量。研究发现,靠近肿瘤侵袭部位的肠系膜神经丛明显萎缩,显示出 Gal 免疫反应(Gal-Ir),与此同时,与肿瘤相邻的外侧肌组织中的 Gal 含量高于肿瘤远处的组织。在进一步的研究中,我们首次通过 IHC 技术证明了 GalR1-3 受体在 CRC 肿瘤和结肠粘膜中的存在,并发现肿瘤组织中较高的 GalR3-Ir 与 CRC 患者较长的总生存期相关。此外,我们还发现,肿瘤附近黏膜下神经丛中较低的 GalR1 表达与 CRC 患者较好的预后相关。这些发现表明,GalR1 可被视为 CRC 的新型治疗靶点。总之,我们的形态学研究提供了记录 Gal 及其受体参与 CRC 进展的新数据,并显示了 IHC 技术对 CRC 患者预后的有用性。
The role of galanin in the progression and prognosis of colorectal cancer: the unfinished story
The paper presents a summary of immunohistochemical (IHC) and biochemical investigations on the presence of galanin (Gal), one of the neuropeptides abundant in the enteric nervous systems, and three types of its receptors (GalR1-3) in colorectal cancer (CRC) tissue and non-involved colon wall and their associations with clinical-pathological data of the CRC patients. We were the first to morphologically demonstrate the presence of endogenous Gal in CRC sections and measure its content in homogenates of tumor tissue and dissected compartments of unchanged colon wall. The prominent atrophy of myenteric plexuses displaying Gal immunoreactivity (Gal-Ir) located close to the tumor invasion was found to be accompanied by higher Gal content in the tumor-adjacent muscularis externa than in tumor-distant tissue. In further studies for the first time, we demonstrated by the IHC technique the presence of the GalR1-3 receptors in the CRC tumors and the colon mucosa and found that higher GalR3-Ir in the tumor tissue correlated with longer overall survival of CRC patients. Furthermore, we discovered that lower GalR1 expression in submucosal plexuses located near the tumor correlated with a better prognosis in patients with CRC. These findings suggest that GalR1 could be considered as a novel therapeutic target in CRC. In conclusion, our morphological investigations provided novel data documenting the involvement of Gal and its receptors in the progression of CRC and showed the usefulness of the IHC technique for the prognosis of CRC patients.
期刊介绍:
The Journal publishes original papers concerning investigations by histochemical and immunohistochemical methods, and performed with the aid of light, super-resolution and electron microscopy, cytometry and imaging techniques. Coverage extends to:
functional cell and tissue biology in animals and plants;
cell differentiation and death;
cell-cell interaction and molecular trafficking;
biology of cell development and senescence;
nerve and muscle cell biology;
cellular basis of diseases.
The histochemical approach is nowadays essentially aimed at locating molecules in the very place where they exert their biological roles, and at describing dynamically specific chemical activities in living cells. Basic research on cell functional organization is essential for understanding the mechanisms underlying major biological processes such as differentiation, the control of tissue homeostasis, and the regulation of normal and tumor cell growth. Even more than in the past, the European Journal of Histochemistry, as a journal of functional cytology, represents the venue where cell scientists may present and discuss their original results, technical improvements and theories.