{"title":"使用一个样本测量树环宽度、密度、元素组成以及稳定碳和氧同位素的方法","authors":"Chenxi Xu, Yaru Zhao, Wenling An, Qingyu Zhao, Yucheng Liu, Masaki Sano, Takeshi Nakatsuka","doi":"10.1007/s11676-024-01707-9","DOIUrl":null,"url":null,"abstract":"<p>Tree-ring width (RW), density, elemental composition, and stable carbon and oxygen isotope (δ<sup>13</sup>C, δ<sup>18</sup>O) are widely used as proxies to assess climate change, ecology, and environmental pollution; however, a specific pretreatment has been needed for each proxy. Here, we developed a method by which each proxy can be measured in the same sample. First, the sample is polished for ring width measurement. After obtaining the ring width data, the sample is cut to form a 1-mm-thick wood plate. The sample is then mounted in a vertical sample holder, and gradually scanned by an X-ray beam. Simultaneously, the count rates of the fluorescent photons of elements (for chemical characterization) and a radiographic grayscale image (for wood density) are obtained, i.e. the density and the element content are obtained. Then, cellulose is isolated from the 1-mm wood plate by removal of lignin, and hemicellulose. After producing this cellulose plate, cellulose subsamples are separated by knife under the microscope for inter-annual and intra-annual stable carbon and oxygen isotope (δ<sup>13</sup>C, δ<sup>18</sup>O) analysis. Based on this method, RW, density, elemental composition, δ<sup>13</sup>C, and δ<sup>18</sup>O can be measured from the same sample, which reduces sample amount and treatment time, and is helpful for multi-proxy comparison and combination research.</p>","PeriodicalId":15830,"journal":{"name":"Journal of Forestry Research","volume":null,"pages":null},"PeriodicalIF":3.4000,"publicationDate":"2024-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Method to measure tree-ring width, density, elemental composition, and stable carbon and oxygen isotopes using one sample\",\"authors\":\"Chenxi Xu, Yaru Zhao, Wenling An, Qingyu Zhao, Yucheng Liu, Masaki Sano, Takeshi Nakatsuka\",\"doi\":\"10.1007/s11676-024-01707-9\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Tree-ring width (RW), density, elemental composition, and stable carbon and oxygen isotope (δ<sup>13</sup>C, δ<sup>18</sup>O) are widely used as proxies to assess climate change, ecology, and environmental pollution; however, a specific pretreatment has been needed for each proxy. Here, we developed a method by which each proxy can be measured in the same sample. First, the sample is polished for ring width measurement. After obtaining the ring width data, the sample is cut to form a 1-mm-thick wood plate. The sample is then mounted in a vertical sample holder, and gradually scanned by an X-ray beam. Simultaneously, the count rates of the fluorescent photons of elements (for chemical characterization) and a radiographic grayscale image (for wood density) are obtained, i.e. the density and the element content are obtained. Then, cellulose is isolated from the 1-mm wood plate by removal of lignin, and hemicellulose. After producing this cellulose plate, cellulose subsamples are separated by knife under the microscope for inter-annual and intra-annual stable carbon and oxygen isotope (δ<sup>13</sup>C, δ<sup>18</sup>O) analysis. Based on this method, RW, density, elemental composition, δ<sup>13</sup>C, and δ<sup>18</sup>O can be measured from the same sample, which reduces sample amount and treatment time, and is helpful for multi-proxy comparison and combination research.</p>\",\"PeriodicalId\":15830,\"journal\":{\"name\":\"Journal of Forestry Research\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.4000,\"publicationDate\":\"2024-03-08\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Forestry Research\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1007/s11676-024-01707-9\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"FORESTRY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Forestry Research","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1007/s11676-024-01707-9","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"FORESTRY","Score":null,"Total":0}
引用次数: 0
摘要
树环宽度(RW)、密度、元素组成以及稳定碳和氧同位素(δ13C、δ18O)被广泛用作评估气候变化、生态学和环境污染的代用指标;然而,每种代用指标都需要进行特定的预处理。在此,我们开发了一种方法,可在同一样本中测量每种代用指标。首先,对样本进行抛光,以便测量环宽。获得环宽数据后,将样品切割成 1 毫米厚的木板。然后将样品安装在垂直样品支架上,用 X 射线光束逐步扫描。同时,获得元素荧光光子的计数率(用于化学特征)和射线灰度图像(用于木材密度),即获得密度和元素含量。然后,通过去除木质素和半纤维素,从 1 毫米的木板中分离出纤维素。制成纤维素板后,在显微镜下用刀将纤维素子样分离出来,进行年际和年内稳定碳和氧同位素(δ13C、δ18O)分析。基于这种方法,可以从同一样品中测量 RW、密度、元素组成、δ13C 和 δ18O,减少了样品量和处理时间,有利于多代理比较和组合研究。
Method to measure tree-ring width, density, elemental composition, and stable carbon and oxygen isotopes using one sample
Tree-ring width (RW), density, elemental composition, and stable carbon and oxygen isotope (δ13C, δ18O) are widely used as proxies to assess climate change, ecology, and environmental pollution; however, a specific pretreatment has been needed for each proxy. Here, we developed a method by which each proxy can be measured in the same sample. First, the sample is polished for ring width measurement. After obtaining the ring width data, the sample is cut to form a 1-mm-thick wood plate. The sample is then mounted in a vertical sample holder, and gradually scanned by an X-ray beam. Simultaneously, the count rates of the fluorescent photons of elements (for chemical characterization) and a radiographic grayscale image (for wood density) are obtained, i.e. the density and the element content are obtained. Then, cellulose is isolated from the 1-mm wood plate by removal of lignin, and hemicellulose. After producing this cellulose plate, cellulose subsamples are separated by knife under the microscope for inter-annual and intra-annual stable carbon and oxygen isotope (δ13C, δ18O) analysis. Based on this method, RW, density, elemental composition, δ13C, and δ18O can be measured from the same sample, which reduces sample amount and treatment time, and is helpful for multi-proxy comparison and combination research.
期刊介绍:
The Journal of Forestry Research (JFR), founded in 1990, is a peer-reviewed quarterly journal in English. JFR has rapidly emerged as an international journal published by Northeast Forestry University and Ecological Society of China in collaboration with Springer Verlag. The journal publishes scientific articles related to forestry for a broad range of international scientists, forest managers and practitioners.The scope of the journal covers the following five thematic categories and 20 subjects:
Basic Science of Forestry,
Forest biometrics,
Forest soils,
Forest hydrology,
Tree physiology,
Forest biomass, carbon, and bioenergy,
Forest biotechnology and molecular biology,
Forest Ecology,
Forest ecology,
Forest ecological services,
Restoration ecology,
Forest adaptation to climate change,
Wildlife ecology and management,
Silviculture and Forest Management,
Forest genetics and tree breeding,
Silviculture,
Forest RS, GIS, and modeling,
Forest management,
Forest Protection,
Forest entomology and pathology,
Forest fire,
Forest resources conservation,
Forest health monitoring and assessment,
Wood Science and Technology,
Wood Science and Technology.