化学灭活传染性液体生物废物的效果:瑞士研究实验室随机抽样研究

Q1 Social Sciences
Alina C. Teuscher , Charlotte Ruhnau , Nicole Stöcklin , Fabienne Wichmann , Evelyn Ilg Hampe , Claudia Bagutti
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引用次数: 0

摘要

实验室废水被认为是微生物从研究环境中逃逸的重要途径。造成实验室生物无意释放的原因可能是在处理传染性液体生物废物(LBW)方面存在缺陷以及灭活程序不当。我们开发了一种分析方法来调查瑞士研究实验室中化学灭活(CI)程序的使用情况,具体做法是现场随机取样,检测可能已灭活的传染性液态生物废料中是否存在传染性慢病毒(HIV-1)和腺病毒(AdV)。此外,我们还收集并评估了 CI 的标准操作程序 (SOP),并通过问卷调查评估了实验室工作人员对 CI 流程的了解程度。虽然我们发现实验室工作人员在枸杞子的 CI 技术知识和培训方面存在一些不足(27 份收回的调查问卷记录了这些不足),但在收集到的 8 份枸杞子样本中没有检测到传染性病毒。虽然我们承认枸杞样品和标准操作程序的数量较少,但我们得出结论认为,对含有传染性慢病毒和腺病毒的枸杞进行CI不会导致瑞士研究实验室向环境中系统性地释放大量传染性病毒。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effectiveness of chemical inactivation of infectious liquid biological waste: A randomized sample study of research laboratories in Switzerland

Laboratory wastewater has been suggested as an important escape route for microorganisms from research environments. Likely reasons for the unintentional release of laboratory organisms are shortcomings in the handling of infectious liquid biological waste (LBW) and inadequate inactivation procedures. We developed an analytical approach to investigate the use of chemical inactivation (CI) procedures in Swiss research laboratories by on-site random sampling of presumably inactivated infectious LBW and testing it for the presence of infectious lentiviruses (HIV-1) and adenoviruses (AdV). In addition, standard operating procedures (SOPs) for CI were collected and evaluated, and laboratory-staff knowledge of CI processes was assessed using a questionnaire. Although we found several deficiencies in the technical knowledge and training of laboratory staff on the CI of LBW, as documented by 27 returned questionnaires, no infectious viruses were detected in the eight LBW samples collected. Whilst we acknowledge that the number of LBW samples and SOPs is small, we conclude that CI of LBW containing infectious lentiviruses and adenoviruses does not result in the systematic release of considerable amounts of infectious viruses into the environment from research laboratories in Switzerland.

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来源期刊
Journal of Biosafety and Biosecurity
Journal of Biosafety and Biosecurity Social Sciences-Linguistics and Language
CiteScore
6.00
自引率
0.00%
发文量
20
审稿时长
41 days
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