方便的预分离方法取决于负电荷，适用于糖测序分析。

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-05-22 DOI:10.1093/bbb/zbae030

Shigemitsu Segawa, Shunji Natsuka

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引用次数: 0

摘要

为了对复杂的聚糖进行精确的结构分析，有必要在液相色谱-质谱（LC-MS）分析之前根据酸度对聚糖进行分馏。传统的高效液相色谱法（HPLC）分馏耗时且通量不高。因此，我们开发了一种使用小型滤芯柱进行快速分馏的方法。这样就能快速处理多个样品，进行聚糖测序。

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Convenient pre-separation method depends on negative charges suitable for glycan sequencing analysis.

For precise structural analysis of complex glycans, it is necessary to fractionate glycans based on acidity prior to liquid chromatography-mass spectrometry analysis. Conventional fractionation using high-performance liquid chromatography is time consuming and not high throughput. Therefore, we developed a rapid method for the fractionation using a small cartridge column. This enables rapid multi-sample processing for glycan sequencing.

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.