方便的预分离方法取决于负电荷，适用于糖测序分析。

IF 1.4 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioscience, Biotechnology, and Biochemistry Pub Date : 2024-05-22 DOI:10.1093/bbb/zbae030

Shigemitsu Segawa, Shunji Natsuka

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引用次数: 0

摘要

为了对复杂的聚糖进行精确的结构分析，有必要在液相色谱-质谱（LC-MS）分析之前根据酸度对聚糖进行分馏。传统的高效液相色谱法（HPLC）分馏耗时且通量不高。因此，我们开发了一种使用小型滤芯柱进行快速分馏的方法。这样就能快速处理多个样品，进行聚糖测序。

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Convenient pre-separation method depends on negative charges suitable for glycan sequencing analysis.

For precise structural analysis of complex glycans, it is necessary to fractionate glycans based on acidity prior to liquid chromatography-mass spectrometry analysis. Conventional fractionation using high-performance liquid chromatography is time consuming and not high throughput. Therefore, we developed a rapid method for the fractionation using a small cartridge column. This enables rapid multi-sample processing for glycan sequencing.

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来源期刊

Bioscience, Biotechnology, and Biochemistry 生物-生化与分子生物学

CiteScore

3.50

自引率

0.00%

发文量

183

审稿时长

1 months

期刊介绍： Bioscience, Biotechnology, and Biochemistry publishes high-quality papers providing chemical and biological analyses of vital phenomena exhibited by animals, plants, and microorganisms, the chemical structures and functions of their products, and related matters. The Journal plays a major role in communicating to a global audience outstanding basic and applied research in all fields subsumed by the Japan Society for Bioscience, Biotechnology, and Agrochemistry (JSBBA).