在日粮中添加甜菜碱,可通过AAT/mTOR途径减轻生长兔在夏季高温期间的肠道损伤。

IF 6.3 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Zimei Li, Junning Pu, Xiang Chen, Yanbin Chen, Xiaoyan Peng, Jingyi Cai, Gang Jia, Hua Zhao, Gang Tian
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引用次数: 0

摘要

背景:本实验的目的是研究在日粮中添加不同水平的甜菜碱(Bet)对夏季高温下生长兔肠道健康的影响。将100只35日龄、体重为748.61±38.59 g的断奶七星肉兔随机分为5个处理组:对照组(基础日粮)和甜菜碱组(基础日粮+0.75、1.0、1.5或2.0 g/kg Bet)。实验期间兔舍的日平均温度为 30.48 °C,相对湿度为 69.44%:结果:日粮中添加 Bet 对生长兔的生长性能和健康状况没有显著影响(P > 0.05),但在夏季高温条件下,与对照组相比,它能提高回肠分泌型免疫球蛋白 A 的含量(P 结论:日粮中添加 Bet 对生长兔的生长性能和健康状况没有显著影响(P > 0.05):膳食中添加 Bet 可通过 AAT/mTOR 途径上调抗炎因子的表达,改善肠道免疫功能,减轻夏季高温对生长兔肠道的损伤,提高肠道健康水平。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Betaine addition to the diet alleviates intestinal injury in growing rabbits during the summer heat through the AAT/mTOR pathway.

Background: The aim of this experiment was to investigate the effect of different levels of betaine (Bet) inclusion in the diet on the intestinal health of growing rabbits under summer heat. A total of 100 weaned Qixing meat rabbits aged 35 d with body weight of 748.61 ± 38.59 g were randomly divided into 5 treatment groups: control group (basal diet) and Bet groups (basal diet + 0.75, 1.0, 1.5 or 2.0 g/kg Bet). The average daily temperature in the rabbitry during the experiment was 30.48 °C and the relative humidity was 69.44%.

Results: Dietary addition of Bet had no significant effect on growth performance and health status of growing rabbits (P > 0.05), but it increased ileal secretory immunoglobulin A content compared to the control under summer heat (P < 0.05). Addition of 0.75 g/kg Bet up-regulated jejunal IL-4, down-regulated ileal TNF-α expression (P < 0.05). The addition of 1.0 g/kg Bet increased the villi height (VH) in the jejunum (P < 0.05). Serum glucose levels were reduced, and the expression of SLC6A20 was up-regulated in jejunum and ileum of rabbits fed with 1.5 g/kg Bet (P < 0.05). When added at 2.0 g/kg, Bet reduced serum HSP70 content, increased jejunal VH, and up-regulated duodenal SLC7A6, SLC38A2, mTOR and 4EBP-2 expression (P < 0.05). Correlation analysis revealed that intestinal mTOR expression was significantly and positively correlated with SLC7A6, SLC38A2, SLC36A1 and IL-4 expression (P < 0.05).

Conclusions: Dietary addition of Bet can up-regulate the expression of anti-inflammatory factors through the AAT/mTOR pathway, improve the intestinal immune function, alleviate intestinal damage in growing rabbits caused by summer heat, and improve intestinal health.

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CiteScore
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