Qibing Gu, Xiayu Zhu, Qiankun Bai, Chengyuan Ji, Yue Zhang, Jiale Ma, Huochun Yao, Zihao Pan
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引用次数: 0
摘要
毒素-抗毒素(TA)系统普遍存在于细菌和古细菌中,在细菌生理学中发挥着多种作用,并被认为在应激适应方面具有重要意义。尽管对各种细菌中的许多 TA 系统进行了广泛的表征,但对这些系统在()内部的研究仍然有限。在此,我们利用 TAfinder 系统分析了 GenBank 数据库中 95 个基因组的 II 型 TA 系统。共检索到 612 个推定的 II 型 TA 系统,并通过系统发育分析将其分为 10 类。值得注意的是,在重要的流行血清型 2、4、5、9、14、Chz、NCL1 和 NCL3 菌株中,这些 TA 系统的出现率较高。接下来的研究利用两种策略确定了 TA 系统的活性,并通过测量 β-半乳糖苷酶活性和转录变化证实了 HigBA 对 VII 型分泌系统的调控作用。此外,我们还揭示了一种迄今为止尚未定性的、高度流行的新型 TA 系统,它由抗毒素-毒素-抗毒素(SS-ATA)组成,调控下游的双组分信号系统。总之,这项研究系统地分析了Ⅴ类动物体内的II型TA系统,强调了HigBA和SS-ATA作为Ⅴ类动物体内重要调控元件的广泛分布。
Bioinformatics analysis of type II toxin-antitoxin systems and regulatory functional assessment of HigBA and SS-ATA in Streptococcus suis
Toxin-antitoxin (TA) systems, which are prevalent in bacteria and archaea, play diverse roles in bacterial physiology and have been proposed to be significant in stress adaptation. Despite the extensive characterization of numerous TA systems in various bacteria, the investigation of these systems within () is still limited. Here, we systematically analyzed the type II TA systems of 95 genomes available in the GenBank database using TAfinder. A total of 612 putative type II TA systems were retrieved and classified into 10 categories by phylogenetic analysis. Notably, an elevated occurrence of these TA systems was observed among the important prevalent serotypes 2, 4, 5, 9, 14, Chz, NCL1, and NCL3 strains. The following study identified the activities of TA systems using two strategies and confirmed the regulatory effect of HigBA on the type VII secretion system in by measuring β-galactosidase activity and transcriptional changes. Moreover, we unveiled a hitherto uncharacterized, highly prevalent novel TA system, with the composition of antitoxin-toxin-antitoxin (SS-ATA), which regulates the downstream two-component signaling system. Altogether, this study systematically analyzed the type II TA systems within , highlighting the widespread distribution of HigBA and SS-ATA as important regulatory elements in .
期刊介绍:
Journal of Integrative Agriculture publishes manuscripts in the categories of Commentary, Review, Research Article, Letter and Short Communication, focusing on the core subjects: Crop Genetics & Breeding, Germplasm Resources, Physiology, Biochemistry, Cultivation, Tillage, Plant Protection, Animal Science, Veterinary Science, Soil and Fertilization, Irrigation, Plant Nutrition, Agro-Environment & Ecology, Bio-material and Bio-energy, Food Science, Agricultural Economics and Management, Agricultural Information Science.