通过机械感应改善调节性 T 细胞的生成。

IF 3.9 3区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of biomedical materials research. Part A Pub Date : 2024-03-07 DOI:10.1002/jbm.a.37702

Lingting Shi, Jee Yoon Lim, Lance C. Kam

{"title":"通过机械感应改善调节性 T 细胞的生成。","authors":"Lingting Shi, Jee Yoon Lim, Lance C. Kam","doi":"10.1002/jbm.a.37702","DOIUrl":null,"url":null,"abstract":"Induced Tregs (iTregs) have great promise in adoptive immunotherapy for treatment of autoimmune diseases. This report investigates the impacts of substrate stiffness on human Treg induction, providing a powerful yet simple approach to improving production of these cells. Conventional CD4+ human T cells were activated on materials of different elastic modulus and cultured under suppressive conditions. Enhanced Treg induction was observed on softer materials as early as 3 days following activation and persisted for multiple weeks. Substrate stiffness also affected epigenetic modification of Treg specific genes and Treg suppressive capacity. Tregs induced on substrates of an optimal stiffness balance quantity and suppressive quality.","PeriodicalId":15142,"journal":{"name":"Journal of biomedical materials research. Part A","volume":"112 7","pages":"1138-1148"},"PeriodicalIF":3.9000,"publicationDate":"2024-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Improving regulatory T cell production through mechanosensing\",\"authors\":\"Lingting Shi, Jee Yoon Lim, Lance C. Kam\",\"doi\":\"10.1002/jbm.a.37702\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Induced Tregs (iTregs) have great promise in adoptive immunotherapy for treatment of autoimmune diseases. This report investigates the impacts of substrate stiffness on human Treg induction, providing a powerful yet simple approach to improving production of these cells. Conventional CD4+ human T cells were activated on materials of different elastic modulus and cultured under suppressive conditions. Enhanced Treg induction was observed on softer materials as early as 3 days following activation and persisted for multiple weeks. Substrate stiffness also affected epigenetic modification of Treg specific genes and Treg suppressive capacity. Tregs induced on substrates of an optimal stiffness balance quantity and suppressive quality.\",\"PeriodicalId\":15142,\"journal\":{\"name\":\"Journal of biomedical materials research. Part A\",\"volume\":\"112 7\",\"pages\":\"1138-1148\"},\"PeriodicalIF\":3.9000,\"publicationDate\":\"2024-03-07\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of biomedical materials research. Part A\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/jbm.a.37702\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"ENGINEERING, BIOMEDICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of biomedical materials research. Part A","FirstCategoryId":"5","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/jbm.a.37702","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENGINEERING, BIOMEDICAL","Score":null,"Total":0}

引用次数: 0

摘要

诱导Tregs（iTregs）在治疗自身免疫性疾病的采用性免疫疗法中大有可为。本报告研究了基底硬度对人类 Treg 诱导的影响，为改善这些细胞的生产提供了一种强大而简单的方法。传统的 CD4+ 人类 T 细胞在不同弹性模量的材料上被激活，并在抑制条件下进行培养。在较软的材料上，早在激活后 3 天就能观察到更强的 Treg 诱导，并能持续数周。基底硬度也会影响 Treg 特异基因的表观遗传修饰和 Treg 抑制能力。在硬度最佳的基质上诱导的 Tregs 在数量和抑制质量上达到了平衡。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Improving regulatory T cell production through mechanosensing

Induced Tregs (iTregs) have great promise in adoptive immunotherapy for treatment of autoimmune diseases. This report investigates the impacts of substrate stiffness on human Treg induction, providing a powerful yet simple approach to improving production of these cells. Conventional CD4⁺ human T cells were activated on materials of different elastic modulus and cultured under suppressive conditions. Enhanced Treg induction was observed on softer materials as early as 3 days following activation and persisted for multiple weeks. Substrate stiffness also affected epigenetic modification of Treg specific genes and Treg suppressive capacity. Tregs induced on substrates of an optimal stiffness balance quantity and suppressive quality.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Journal of biomedical materials research. Part A 工程技术-材料科学：生物材料

CiteScore

10.40

自引率

2.00%

发文量

135

审稿时长

3.6 months

期刊介绍： The Journal of Biomedical Materials Research Part A is an international, interdisciplinary, English-language publication of original contributions concerning studies of the preparation, performance, and evaluation of biomaterials; the chemical, physical, toxicological, and mechanical behavior of materials in physiological environments; and the response of blood and tissues to biomaterials. The Journal publishes peer-reviewed articles on all relevant biomaterial topics including the science and technology of alloys,polymers, ceramics, and reprocessed animal and human tissues in surgery,dentistry, artificial organs, and other medical devices. The Journal also publishes articles in interdisciplinary areas such as tissue engineering and controlled release technology where biomaterials play a significant role in the performance of the medical device. The Journal of Biomedical Materials Research is the official journal of the Society for Biomaterials (USA), the Japanese Society for Biomaterials, the Australasian Society for Biomaterials, and the Korean Society for Biomaterials. Articles are welcomed from all scientists. Membership in the Society for Biomaterials is not a prerequisite for submission.