鉴定暴露于表面活性剂的永生化人类角质细胞 HaCaT 细胞系中转化系统的蛋白质成分。

Q3 Biochemistry, Genetics and Molecular Biology

Biomeditsinskaya khimiya Pub Date : 2024-02-01 DOI:10.18097/PBMC20247001061

T S Shkrigunov, N E Vavilov, N F Samenkova, Yu S Kisrieva, A L Rusanov, D D Romashin, I I Karuzina, A V Lisitsa, N A Petushkova

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引用次数: 0

摘要

我们使用枪式质谱法评估了 HaCat 细胞在十二烷基硫酸钠（阴离子表面活性剂）和 Triton-X100 （非离子表面活性剂）两种浓度（12.5 µg/ml 和 25.0 µg/ml）处理下的蛋白质组变化。研究显示，Triton-X100 诱导了孤儿 CYP2S1（生物转化第一阶段）。我们发现了生物转化第二阶段（谷胱甘肽-S-转移酶，GSTs）和第三阶段（溶质运载蛋白，SLCs）的蛋白质，以及抗氧化蛋白质（过氧化还原酶，PRDXs；过氧化氢酶，CAT；硫氧还原酶，TXN）。因此，所有三个异生物解毒阶段的蛋白质都被检测到了。这些结果为利用 HaCaT 角质细胞作为人体表皮模型来研究药物/毒物在人体皮肤中的体外代谢提供了新的前景。

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Identification of protein components of the transformation system in the cell line of immortalized human keratinocytes HaCaT exposed to surfactants.

Using the method of shotgun mass spectrometry, we have evaluated changes in the proteomic profile of HaCat cells in response to the treatment with sodium dodecyl sulfate (anionic surfactant) and Triton-X100 (non-ionic surfactant) in two concentrations (12.5 µg/ml and 25.0 µg/ml). The study revealed induction of orphan CYP2S1 (biotransformation phase I) in response to Triton-X100. We have identified proteins of II (glutathione-S-transferases, GSTs) and III (solute carrier proteins, SLCs) biotransformation phases, as well as antioxidant proteins (peroxiredoxins, PRDXs; catalase, CAT; thioredoxin, TXN). Thus, proteins of all three xenobiotic detoxification phases were detected. The presented results suggest a new prospect of using HaCaT keratinocytes as a model of human epidermis for studying the metabolism of drugs/toxicants in human skin in vitro.

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来源期刊

Biomeditsinskaya khimiya Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)

CiteScore

1.30

自引率

0.00%

发文量

期刊介绍： The aim of the Russian-language journal "Biomeditsinskaya Khimiya" (Biomedical Chemistry) is to introduce the latest results obtained by scientists from Russia and other Republics of the Former Soviet Union. The Journal will cover all major areas of Biomedical chemistry, including neurochemistry, clinical chemistry, molecular biology of pathological processes, gene therapy, development of new drugs and their biochemical pharmacology, introduction and advertisement of new (biochemical) methods into experimental and clinical medicine etc. The Journal also publish review articles. All issues of journal usually contain invited reviews. Papers written in Russian contain abstract (in English).