短讯：蚊子组蛋白 2A 蛋白促进日本脑炎病毒在蚊子体内的感染

IF 1.8 4区医学 Q3 INFECTIOUS DISEASES

Vector borne and zoonotic diseases Pub Date : 2024-04-01 Epub Date: 2024-02-26 DOI:10.1089/vbz.2023.0081

Hameed Muddassar, Mengli Chen, Tong Zhang, Nawaz Mohsin, Lei Kang, Jiayang Zheng, Zongjie Li, Donghua Shao, Jianchao Wei, Beibei Li, Yafeng Qiu, Zhiyong Ma, Ke Liu

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引用次数: 0

摘要

日本脑炎病毒主要流行于亚洲和大洋洲的热带和亚热带地区。通过使用针对日本脑炎病毒 E 蛋白的单克隆抗体进行免疫沉淀-质谱分析，我们发现蚊子组蛋白 2A 蛋白能与日本脑炎病毒颗粒结合。在蚊子细胞的唾液腺和上清液中检测到了 H2A 与 JEV 的结合。此外，体外和体内的 RNA 干扰实验证实，H2A 蛋白能促进蚊子感染 JEV。总之，我们发现蚊子 H2A 是一种支持 JEV 感染的因子，有可能促进 JEV 的跨物种传播。

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Short Communication: Mosquito Histone 2A Protein Facilitate Japanese Encephalitis Virus Infection in the Mosquito.

Japanese encephalitis virus is mainly prevalent in the tropical and subtropical regions of Asia and Oceania. Through immunoprecipitation-mass spectrometry analysis using monoclonal antibodies targeting JEV E protein, we found that mosquito Histone 2A protein could bind to JEV particles. The binding of H2A and JEV was detected in the salivary gland and supernatant of mosquito cells. Furthermore, RNA interference experiments in vitro and in vivo confirmed that H2A protein promotes JEV infection in mosquitoes. In summary, we found that mosquito H2A is a factor that supports JEV infection and can potentially facilitate cross-species transmission of JEV.

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来源期刊

Vector borne and zoonotic diseases 医学-传染病学

CiteScore

4.70

自引率

4.80%

发文量

审稿时长

3-8 weeks

期刊介绍： Vector-Borne and Zoonotic Diseases is an authoritative, peer-reviewed journal providing basic and applied research on diseases transmitted to humans by invertebrate vectors or non-human vertebrates. The Journal examines geographic, seasonal, and other risk factors that influence the transmission, diagnosis, management, and prevention of this group of infectious diseases, and identifies global trends that have the potential to result in major epidemics. Vector-Borne and Zoonotic Diseases coverage includes: -Ecology -Entomology -Epidemiology -Infectious diseases -Microbiology -Parasitology -Pathology -Public health -Tropical medicine -Wildlife biology -Bacterial, rickettsial, viral, and parasitic zoonoses