不同溶剂对摩洛哥中阿特拉斯地区小叶紫檀(Micromeria graeca L.)总酚含量、总类黄酮含量、抗氧化和抗真菌活性的影响。

IF 3.4 Q2 BIOCHEMICAL RESEARCH METHODS
Biochemistry Research International Pub Date : 2024-02-26 eCollection Date: 2024-01-01 DOI:10.1155/2024/9027997
Fatima El Kamari, Hajar El Omari, Karima El-Mouhdi, Amina Chlouchi, Anjoud Harmouzi, Ilham Lhilali, Jihane El Amrani, Chadia Zahouani, Zouhair Hajji, Driss Ousaaid
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The antifungal capacity against the two fungal strains (<i>Candida albicans</i> and <i>Aspergillus niger</i>) was performed using the method of diffusion on disc. The dosage of phytochemical compounds revealed that the highest values were established in water extract with values of 360 ± 22.1 mg GAE/g dry weight plant and 81.3 ± 21.2 mg RE/g dry weight plant for TPC and TFC, respectively. In addition, the strongest antioxidant activity measured by DPPH and FRAP assays was established in water extract with IC<sub>50</sub> values of 0.33 ± 0.23 and 0.23 ± 0.12 mg/mL, respectively, while the methanol extract showed the best antioxidant activity as measured by TAC with an IC<sub>50</sub> of 483 ± 17.6 mg GAEq/g dry weight plant. 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引用次数: 0

摘要

Micromeria graeca L.是生物活性化合物(如酚类化合物)的密集化学来源,具有各种与健康相关的特性。本研究旨在探讨不同萃取溶剂对酚和类黄酮含量的影响,以及不同萃取物的抗氧化和抗真菌活性。首先使用三种萃取溶剂(甲醇、乙酸乙酯和水)制备索氏提取物,然后使用三种互补测定法(DPPH、FRAP 和 TAC)检测其多酚含量、类黄酮含量和抗氧化潜力。采用圆盘扩散法检测了两种真菌菌株(白色念珠菌和黑曲霉)的抗真菌能力。植物化学物质的用量表明,水提取物中的植物化学物质含量最高,TPC 和 TFC 的含量分别为 360 ± 22.1 mg GAE/g(干重植株)和 81.3 ± 21.2 mg RE/g(干重植株)。此外,用 DPPH 和 FRAP 法测定,水提取物的抗氧化活性最强,IC50 值分别为 0.33 ± 0.23 和 0.23 ± 0.12 mg/mL,而用 TAC 法测定,甲醇提取物的抗氧化活性最好,IC50 为 483 ± 17.6 mg GAEq/g(干重植物)。水提取物对白色念珠菌具有最重要的抗真菌活性,抑制区为 16 ± 1.6 mm,MFC = 500 μg/mL,而乙酸乙酯提取物对所研究的两种真菌菌株的活性最低。Micromeria graeca L.含有大量生物活性成分,具有很高的抗氧化和抗真菌潜力,这可能使其成为一种很有前景的抗氧化剂和天然抗真菌剂来源。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effects of Different Solvents on the Total Phenol Content, Total Flavonoid Content, Antioxidant, and Antifungal Activities of Micromeria graeca L. from Middle Atlas of Morocco.

Micromeria graeca L. is a dense chemical source of bioactive compounds such as phenolic compounds, which have various health-related properties. The current study aimed to investigate the impact of different extractor solvents on phenol and flavonoid contents, as well as the antioxidant and antifungal activities of different extracts. Initially, three extractor solvents (methanol, ethyl acetate, and water) were used to prepare the Soxhlet extracts, which were then examined for their polyphenolic content, flavonoid content, and antioxidant potential using three complementary assays (DPPH, FRAP, and TAC). The antifungal capacity against the two fungal strains (Candida albicans and Aspergillus niger) was performed using the method of diffusion on disc. The dosage of phytochemical compounds revealed that the highest values were established in water extract with values of 360 ± 22.1 mg GAE/g dry weight plant and 81.3 ± 21.2 mg RE/g dry weight plant for TPC and TFC, respectively. In addition, the strongest antioxidant activity measured by DPPH and FRAP assays was established in water extract with IC50 values of 0.33 ± 0.23 and 0.23 ± 0.12 mg/mL, respectively, while the methanol extract showed the best antioxidant activity as measured by TAC with an IC50 of 483 ± 17.6 mg GAEq/g dry weight plant. The water extract recorded the most important antifungal activity against Candida albicans with an inhibition zone of 16 ± 1.6 mm and MFC = 500 μg/mL, whereas ethyl acetate extract showed the lowest activity against both studied fungi strains. Micromeria graeca L. contains considerable amounts of bioactive contents with high antioxidant and antifungal potentials, which may make it a promising source of antioxidants and natural antifungal agents.

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来源期刊
Biochemistry Research International
Biochemistry Research International BIOCHEMICAL RESEARCH METHODS-
CiteScore
6.30
自引率
0.00%
发文量
27
审稿时长
14 weeks
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