暴露于硅钙钛矿和钠长石生物陶瓷的 MC3T3-E1 前成骨细胞的矿物基质沉积:与羟基磷灰石的体外比较。

IF 3.9 3区 医学 Q2 ENGINEERING, BIOMEDICAL
July Andrea Rincón-López, Jennifer Andrea Hermann-Muñoz, Rainer Detsch, Raúl Rangel-López, Juan Muñoz-Saldaña, Sergio Jiménez-Sandoval, Juan Manuel Alvarado-Orozco, Aldo R. Boccaccini
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引用次数: 0

摘要

本研究介绍了硅钙钛石(SC)和纳格施密特石(Nagel)相对体外成骨的影响。已知的生物源羟基磷灰石(BHAp)被用作骨诱导特性的标准。评估在传统和成骨培养基中进行,以比较评估生物陶瓷的成骨能力。首先,评估了培养 24 小时、7 天和 14 天后材料对细胞活力的影响。此外,还通过荧光显微镜观察了细胞形态和在致密生物陶瓷表面的附着情况。具体来说,碱性磷酸酶(ALP)活性被作为骨细胞分化早期阶段的成骨标志物进行了评估。通过磷酸钙沉积和细胞外囊泡的形成,观察到细胞外基质矿化。此外,还通过扫描电子显微镜确定了细胞表型。这些结果提供了培养 7 天和 14 天后细胞附着、增殖和成骨分化过程的相关信息。结果表明,SC 相和 Nagel 相能促进细胞增殖和分化,而 Nagel 相则表现出更优越的成骨性,与 SC 相和 BHAp 相比,它能在更大程度上促进 MC3T3-E1 细胞的分化,这体现在传统培养基和成骨培养基都能在更短的时间内获得更多的沉积物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Mineral matrix deposition of MC3T3-E1 pre-osteoblastic cells exposed to silicocarnotite and nagelschmidtite bioceramics: In vitro comparison to hydroxyapatite

Mineral matrix deposition of MC3T3-E1 pre-osteoblastic cells exposed to silicocarnotite and nagelschmidtite bioceramics: In vitro comparison to hydroxyapatite

This work presents the effect of the silicocarnotite (SC) and nagelschmidtite (Nagel) phases on in vitro osteogenesis. The known hydroxyapatite of biological origin (BHAp) was used as a standard of osteoconductive characteristics. The evaluation was carried out in conventional and osteogenic media for comparative purposes to assess the osteogenic ability of the bioceramics. First, the effect of the material on cell viability at 24 h, 7 and 14 days of incubation was evaluated. In addition, cell morphology and attachment on dense bioceramic surfaces were observed by fluorescence microscopy. Specifically, alkaline phosphatase (ALP) activity was evaluated as an osteogenic marker of the early stages of bone cell differentiation. Mineralized extracellular matrix was observed by calcium phosphate deposits and extracellular vesicle formation. Furthermore, cell phenotype determination was confirmed by scanning electron microscope. The results provided relevant information on the cell attachment, proliferation, and osteogenic differentiation processes after 7 and 14 days of incubation. Finally, it was demonstrated that SC and Nagel phases promote cell proliferation and differentiation, while the Nagel phase exhibited a superior osteoconductive behavior and could promote MC3T3-E1 cell differentiation to a higher extent than SC and BHAp, which was reflected in a higher number of deposits in a shorter period for both conventional and osteogenic media.

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来源期刊
Journal of biomedical materials research. Part A
Journal of biomedical materials research. Part A 工程技术-材料科学:生物材料
CiteScore
10.40
自引率
2.00%
发文量
135
审稿时长
3.6 months
期刊介绍: The Journal of Biomedical Materials Research Part A is an international, interdisciplinary, English-language publication of original contributions concerning studies of the preparation, performance, and evaluation of biomaterials; the chemical, physical, toxicological, and mechanical behavior of materials in physiological environments; and the response of blood and tissues to biomaterials. The Journal publishes peer-reviewed articles on all relevant biomaterial topics including the science and technology of alloys,polymers, ceramics, and reprocessed animal and human tissues in surgery,dentistry, artificial organs, and other medical devices. The Journal also publishes articles in interdisciplinary areas such as tissue engineering and controlled release technology where biomaterials play a significant role in the performance of the medical device. The Journal of Biomedical Materials Research is the official journal of the Society for Biomaterials (USA), the Japanese Society for Biomaterials, the Australasian Society for Biomaterials, and the Korean Society for Biomaterials. Articles are welcomed from all scientists. Membership in the Society for Biomaterials is not a prerequisite for submission.
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