John Quiroga , Bayron Cortes , José Sarmiento , Gabriel Morán , Claudio Henríquez
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引用次数: 0
摘要
这项研究探讨了马中性粒细胞胞外捕获物(NET)的形成,NET 是消除感染的关键,与各种马炎症疾病有关。我们研究了马中性粒细胞在刺激下释放 NET 的分子途径。我们使用了 PMA、A23187、LPS、PAF、OZ 和细胞因子,观察到中性粒细胞对 PMA、PAF 和 A23187 的释放反应。相反,LPS、OZ 和测试的细胞因子没有诱导 DNA 释放,也没有持续诱导组蛋白 4 的瓜氨酸化。肽基精氨酸脱氨酶抑制完全阻止了NET的释放,而NADPH氧化酶和线粒体活性氧只在PMA诱导的NET中起作用。抑制中性粒细胞弹性蛋白酶会适度影响 PAF 诱导的 NET 释放,但不会影响 PMA 或 A23187 诱导的 NET,而髓过氧化物酶不会促进 NET 释放。我们希望为今后研究 NET 在马健康和疾病中的作用以及寻找潜在治疗靶点奠定基础。
Characterization of extracellular trap production and release by equine neutrophils in response to different stimuli
This study explores Neutrophil Extracellular Trap (NET) formation in equine neutrophils, which is crucial for eliminating infections and is implicated in various equine inflammatory diseases. We investigated the molecular pathways involved in NET release by equine neutrophils in response to stimuli. We use PMA, A23187, LPS, PAF, OZ, and cytokines, observing NET release in response to PMA, PAF, and A23187. In contrast, LPS, OZ, and the cytokines tested did not induce DNA release or did not consistently induce citrullination of histone 4. Peptidyl-arginine deiminase inhibition completely halted NET release, while NADPH oxidase and mitochondrial reactive oxygen species only played a role in PMA-induced NETs. Neutrophil elastase inhibition modestly affected PAF-induced NET liberation but not in PMA or A23187-induced NET, while myeloperoxidase did not contribute to NET release. We expect to provide a foundation for future investigations into the role of NETs in equine health and disease and the search for potential therapeutic targets.
期刊介绍:
Developmental and Comparative Immunology (DCI) is an international journal that publishes articles describing original research in all areas of immunology, including comparative aspects of immunity and the evolution and development of the immune system. Manuscripts describing studies of immune systems in both vertebrates and invertebrates are welcome. All levels of immunological investigations are appropriate: organismal, cellular, biochemical and molecular genetics, extending to such fields as aging of the immune system, interaction between the immune and neuroendocrine system and intestinal immunity.