{"title":"掺入纳米羟基磷灰石的玻璃离子粘结剂在正畸带下对变异链球菌和乳酸杆菌的抗菌特性：活体分口研究。","authors":"Maryam Shirazi, Aydin Pirzeh, Marzieh Atashgaran","doi":"","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Fixed orthodontic appliances enhance dental plaque accumulation. Glass ionomer (GI) is among the most popular orthodontic cement. It possesses antibacterial properties; however, its antibacterial activity may not be sufficient for caries prevention. Although evidence shows that the addition of 8wt% nano-hydroxyapatite (nHA) may enhance the antibacterial properties of GI, no clinical study has been conducted in this respect. Thus, this study aimed to assess the subgingival accumulation of <i>Streptococcus mutans</i> (S. mutans) and <i>Lactobacillus acidophilus</i> (<i>L. acidophilus</i>) around orthodontic bands cemented with conventional GI and GI reinforced with 8wt% nHA.</p><p><strong>Materials and methods: </strong>This split-mouth clinical trial was conducted on 20 patients requiring a lingual arch. The patients were randomly assigned to two groups. In group 1, the right molar band was cemented with pure Fuji I (GC), and the left was cemented with Fuji I containing 8wt% nHA. In group 2, the right molar band was cemented with Fuji I containing 8wt% nHA, and the left was cemented with Fuji I. After 3 months, subgingival sampling was performed by sterile paper points. <i>S. mutans</i> and <i>L. acidophilus</i> were cultured on MSB and MRS agar, and colonies were counted by a colony counter. Data were analyzed by independent samples <i>t</i>-test using SPSS 25 at a 0.05 level of significance.</p><p><strong>Results: </strong>The mean counts of <i>S. mutans</i>, aerobic and anaerobic lactobacilli, and total bacterial around orthodontic bands cemented with Fuji I containing 8wt% nHA were significantly lower than those around orthodontic bands cemented with pure Fuji I (<i>P</i> < 0.05).</p><p><strong>Conclusion: </strong>The addition of 8wt% nHA to GI cement can enhance its antibacterial properties for the cementation of orthodontic bands, decrease the accumulation of cariogenic bacteria, and probably decrease the incidence of caries in orthodontic patients.</p>","PeriodicalId":11016,"journal":{"name":"Dental Research Journal","volume":"21 ","pages":"1"},"PeriodicalIF":0.0000,"publicationDate":"2024-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10899160/pdf/","citationCount":"0","resultStr":"{\"title\":\"Antimicrobial properties of glass-ionomer cement incorporated with nano-hydroxyapatite against mutans streptococci and lactobacilli under orthodontic bands: An <i>in vivo</i> split-mouth study.\",\"authors\":\"Maryam Shirazi, Aydin Pirzeh, Marzieh Atashgaran\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Fixed orthodontic appliances enhance dental plaque accumulation. Glass ionomer (GI) is among the most popular orthodontic cement. It possesses antibacterial properties; however, its antibacterial activity may not be sufficient for caries prevention. Although evidence shows that the addition of 8wt% nano-hydroxyapatite (nHA) may enhance the antibacterial properties of GI, no clinical study has been conducted in this respect. Thus, this study aimed to assess the subgingival accumulation of <i>Streptococcus mutans</i> (S. mutans) and <i>Lactobacillus acidophilus</i> (<i>L. acidophilus</i>) around orthodontic bands cemented with conventional GI and GI reinforced with 8wt% nHA.</p><p><strong>Materials and methods: </strong>This split-mouth clinical trial was conducted on 20 patients requiring a lingual arch. The patients were randomly assigned to two groups. In group 1, the right molar band was cemented with pure Fuji I (GC), and the left was cemented with Fuji I containing 8wt% nHA. In group 2, the right molar band was cemented with Fuji I containing 8wt% nHA, and the left was cemented with Fuji I. After 3 months, subgingival sampling was performed by sterile paper points. <i>S. mutans</i> and <i>L. acidophilus</i> were cultured on MSB and MRS agar, and colonies were counted by a colony counter. Data were analyzed by independent samples <i>t</i>-test using SPSS 25 at a 0.05 level of significance.</p><p><strong>Results: </strong>The mean counts of <i>S. mutans</i>, aerobic and anaerobic lactobacilli, and total bacterial around orthodontic bands cemented with Fuji I containing 8wt% nHA were significantly lower than those around orthodontic bands cemented with pure Fuji I (<i>P</i> < 0.05).</p><p><strong>Conclusion: </strong>The addition of 8wt% nHA to GI cement can enhance its antibacterial properties for the cementation of orthodontic bands, decrease the accumulation of cariogenic bacteria, and probably decrease the incidence of caries in orthodontic patients.</p>\",\"PeriodicalId\":11016,\"journal\":{\"name\":\"Dental Research Journal\",\"volume\":\"21 \",\"pages\":\"1\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-01-25\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10899160/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Dental Research Journal\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q2\",\"JCRName\":\"Dentistry\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Dental Research Journal","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"Dentistry","Score":null,"Total":0}
引用次数: 0
摘要
背景：固定正畸装置会增加牙菌斑的积累。玻璃离聚体（GI）是最流行的正畸粘结剂之一。它具有抗菌特性，但其抗菌活性可能不足以预防龋齿。虽然有证据表明添加 8wt% 的纳米羟基磷灰石（nHA）可以增强玻璃离子粘合剂的抗菌性能，但目前还没有这方面的临床研究。因此，本研究旨在评估变异链球菌（S. mutans）和嗜酸乳杆菌（L. acidophilus）在用传统 GI 和 8wt% 纳米羟基磷灰石（nHA）增强的 GI 粘结的正畸带周围的龈下积聚情况：这项分口临床试验是针对 20 名需要舌侧牙弓的患者进行的。患者被随机分配到两组。在第一组中，右侧磨牙带用纯富士I（GC）粘结，左侧磨牙带用含8wt% nHA的富士I粘结。3 个月后，用无菌纸点进行龈下取样。在 MSB 和 MRS 琼脂上培养变异杆菌和嗜酸乳杆菌，并用菌落计数器对菌落进行计数。数据采用 SPSS 25 进行独立样本 t 检验，显著性水平为 0.05：结果：用含 8wt% nHA 的富士 I 粘结的正畸带周围的变异牙齿螺杆菌、需氧和厌氧乳酸菌以及细菌总数的平均计数显著低于用纯富士 I 粘结的正畸带周围的平均计数（P < 0.05）：结论：在 GI 骨水泥中添加 8wt% 的 nHA 可增强其在粘结正畸带时的抗菌性能，减少致龋细菌的积累，并可能降低正畸患者的龋病发病率。
Antimicrobial properties of glass-ionomer cement incorporated with nano-hydroxyapatite against mutans streptococci and lactobacilli under orthodontic bands: An in vivo split-mouth study.
Background: Fixed orthodontic appliances enhance dental plaque accumulation. Glass ionomer (GI) is among the most popular orthodontic cement. It possesses antibacterial properties; however, its antibacterial activity may not be sufficient for caries prevention. Although evidence shows that the addition of 8wt% nano-hydroxyapatite (nHA) may enhance the antibacterial properties of GI, no clinical study has been conducted in this respect. Thus, this study aimed to assess the subgingival accumulation of Streptococcus mutans (S. mutans) and Lactobacillus acidophilus (L. acidophilus) around orthodontic bands cemented with conventional GI and GI reinforced with 8wt% nHA.
Materials and methods: This split-mouth clinical trial was conducted on 20 patients requiring a lingual arch. The patients were randomly assigned to two groups. In group 1, the right molar band was cemented with pure Fuji I (GC), and the left was cemented with Fuji I containing 8wt% nHA. In group 2, the right molar band was cemented with Fuji I containing 8wt% nHA, and the left was cemented with Fuji I. After 3 months, subgingival sampling was performed by sterile paper points. S. mutans and L. acidophilus were cultured on MSB and MRS agar, and colonies were counted by a colony counter. Data were analyzed by independent samples t-test using SPSS 25 at a 0.05 level of significance.
Results: The mean counts of S. mutans, aerobic and anaerobic lactobacilli, and total bacterial around orthodontic bands cemented with Fuji I containing 8wt% nHA were significantly lower than those around orthodontic bands cemented with pure Fuji I (P < 0.05).
Conclusion: The addition of 8wt% nHA to GI cement can enhance its antibacterial properties for the cementation of orthodontic bands, decrease the accumulation of cariogenic bacteria, and probably decrease the incidence of caries in orthodontic patients.
期刊介绍：
Dental Research Journal, a publication of Isfahan University of Medical Sciences, is a peer-reviewed online journal with Bimonthly print on demand compilation of issues published. The journal’s full text is available online at http://www.drjjournal.net. The journal allows free access (Open Access) to its contents and permits authors to self-archive final accepted version of the articles on any OAI-compliant institutional / subject-based repository. The journal will cover technical and clinical studies related to health, ethical and social issues in field of Dentistry. Articles with clinical interest and implications will be given preference.
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