通过比较不同单体的分子印迹电化学生物传感器检测凝血酶。

IF 1.9 4区医学 Q3 BIOCHEMICAL RESEARCH METHODS

Bioanalysis Pub Date : 2024-03-01 DOI:10.4155/bio-2023-0203

Fatih Turk, Nimet Yildirim-Tirgil

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引用次数: 0

摘要

目的：研究电化学生物传感器中的分子印迹聚合物（MIPs），用于检测凝血酶（一种重要的蛋白质生物标记物）。比较不同的单体，以展示其不同的灵敏度、特异性和稳定性优势。材料与方法：多巴胺、硫氨酸和乙醇胺是合成 MIP 的单体。电化学方法和原子力显微镜表征传感器表面。对其性能进行评估，重点是单体特异性电化学反应。结果：单体特异性电化学反应凸显了多巴胺在 30 天内卓越的信号变化和稳定性。值得注意的是，检测限低至 5 pg/ml，线性范围宽（5-200 pg/ml），对干扰物的选择性增强。结论基于多巴胺的 MIPs 在高性能电化学凝血酶生物传感器中大有可为，有望在临床诊断中得到广泛应用。

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Molecularly imprinted electrochemical biosensor for thrombin detection by comparing different monomers.

Aim: Investigating molecularly imprinted polymers (MIPs) in electrochemical biosensors for thrombin detection, an essential protein biomarker. Comparing different monomers to showcase distinct sensitivity, specificity and stability advantages. Materials & methods: Dopamine, thionine and ethanolamine serve as monomers for MIP synthesis. Electrochemical methods and atomic force microscopy characterize sensor surfaces. Performance is evaluated, emphasizing monomer-specific electrochemical responses. Results: Monomer-specific electrochemical responses highlight dopamine's superior signal change and stability over 30 days. Notably, a low 5 pg/ml limit of detection, a broad linear range (5-200 pg/ml) and enhanced selectivity against interferents are observed. Conclusion: Dopamine-based MIPs show promise for high-performance electrochemical thrombin biosensors, suggesting significant applications in clinical diagnostics.

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来源期刊

Bioanalysis BIOCHEMICAL RESEARCH METHODS-CHEMISTRY, ANALYTICAL

CiteScore

3.30

自引率

16.70%

发文量

审稿时长

2 months

期刊介绍： Reliable data obtained from selective, sensitive and reproducible analysis of xenobiotics and biotics in biological samples is a fundamental and crucial part of every successful drug development program. The same principles can also apply to many other areas of research such as forensic science, toxicology and sports doping testing. The bioanalytical field incorporates sophisticated techniques linking sample preparation and advanced separations with MS and NMR detection systems, automation and robotics. Standards set by regulatory bodies regarding method development and validation increasingly define the boundaries between speed and quality. Bioanalysis is a progressive discipline for which the future holds many exciting opportunities to further reduce sample volumes, analysis cost and environmental impact, as well as to improve sensitivity, specificity, accuracy, efficiency, assay throughput, data quality, data handling and processing. The journal Bioanalysis focuses on the techniques and methods used for the detection or quantitative study of analytes in human or animal biological samples. Bioanalysis encourages the submission of articles describing forward-looking applications, including biosensors, microfluidics, miniaturized analytical devices, and new hyphenated and multi-dimensional techniques. Bioanalysis delivers essential information in concise, at-a-glance article formats. Key advances in the field are reported and analyzed by international experts, providing an authoritative but accessible forum for the modern bioanalyst.