伊朗结直肠癌患者体内长非编码 RNA PCAT1 的上调及其作为潜在诊断生物标记物的作用

IF 1.1 4区 生物学 Q4 GENETICS & HEREDITY
Negin Sadi Khosroshahi, Shabnam Koulaeizadeh, Adel Abdi, Sama Akbarzadeh, Seyed Mehran Hashemi Aghdam, Ali Rajabi, Reza Safaralizadeh
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引用次数: 0

摘要

背景:长非编码 RNA(lncRNA)作为一种关键分子在癌症的发生发展中起着至关重要的作用。在结直肠癌(CRC)中,各种lncRNA与细胞增殖、凋亡、迁移和侵袭有关。LncRNA前列腺癌相关转录本1(PCAT-1)作为一种致癌因子,是一种调节细胞增殖、迁移、侵袭和凋亡的诊断生物标志物。研究方法本研究评估了伊朗患者 PCAT-1、结肠癌发生率和病理特征之间的关系。研究样本包括 100 例伊朗 CRC 患者的结直肠肿瘤组织和 100 例邻近的健康组织。从癌组织和非癌组织中提取 RNA,合成互补 DNA。采用实时 PCR 方法评估 PCAT-1 的表达水平,并使用 SPSS 软件进行数据分析。结果在这项研究中,伊朗患者肿瘤组织中 PCAT-1 的表达水平明显升高,而患者的病理研究与 PCAT-1 的表达谱无明显关系。结论我们的研究结果表明,伊朗患者结直肠肿瘤组织中 PCAT-1 的高表达可作为诊断 CRC 的生物标志物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Upregulation of Long Noncoding RNA PCAT1 in Iranian Patients with Colorectal Cancer and Its Performance as a Potential Diagnostic Biomarker.

Background: Long noncoding RNAs (lncRNAs) as critical molecules play an essential role in the development of cancers. In colorectal cancer (CRC), various lncRNAs are related to cell proliferation, apoptosis, migration, and invasion. LncRNA prostate cancer-associated transcript 1 (PCAT-1), as an oncogenic factor, is a diagnostic biomarker that regulates cell proliferation, migration, invasion, and apoptosis. Methods: This study evaluated the relationship between PCAT-1, CRC occurrence, and pathological features of Iranian patients. The studied samples included 100 colorectal tumor tissues and 100 adjacent healthy tissues of Iranian CRC patients. RNAs were extracted from cancerous and noncancerous tissues to synthesize complementary DNA. The expression level of PCAT-1 was assessed using the real-time PCR method, and the data analysis was assessed using SPSS software. Results: In this study, expression level of PCAT-1 in tumor tissue was significantly increased in Iranian patients, and pathological studies of the patients had no significant relationship with the PCAT-1 expression profile. Conclusion: Our results suggested that the high expression of PCAT-1 resulted in the occurrence of colorectal tumor tissues in Iranian patients, which can be considered a diagnostic biomarker in CRC.

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来源期刊
CiteScore
2.50
自引率
7.10%
发文量
63
审稿时长
1 months
期刊介绍: Genetic Testing and Molecular Biomarkers is the leading peer-reviewed journal covering all aspects of human genetic testing including molecular biomarkers. The Journal provides a forum for the development of new technology; the application of testing to decision making in an increasingly varied set of clinical situations; ethical, legal, social, and economic aspects of genetic testing; and issues concerning effective genetic counseling. This is the definitive resource for researchers, clinicians, and scientists who develop, perform, and interpret genetic tests and their results. Genetic Testing and Molecular Biomarkers coverage includes: -Diagnosis across the life span- Risk assessment- Carrier detection in individuals, couples, and populations- Novel methods and new instrumentation for genetic testing- Results of molecular, biochemical, and cytogenetic testing- Genetic counseling
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