利用罗替尼诱导的大鼠模型研究奥西孟在减少α-突触核蛋白聚集中的作用

Ankul Singh Suresh, Aarita Sood, Chitra Vellapandian
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引用次数: 0

摘要

背景:帕金森病的定义是中脑黑质多巴胺能神经元的丧失,并伴有路易体。其确切机制尚不完全清楚:本研究旨在通过降低鱼藤酮诱导的大鼠模型大脑中的α-突触核蛋白聚集水平,确定奥西美尼是否具有抗帕金森病活性。给大鼠注射载体、对照组(鱼藤酮,2.5 毫克/千克,静注)、标准剂量(左旋多巴,10 毫克/千克,静注)、低剂量(66.66 毫克/千克,静注)、中剂量(100 毫克/千克,静注)和高剂量(200 毫克/千克,静注)试验药物。大鼠的运动能力通过开阔地试验、光度计试验、悬挂钢丝试验和催眠试验进行评估。大鼠脑样本中 AchE、D2 受体和 α- 突触核蛋白等生物标志物的表达水平通过 ELISA 进行了检测。此外,还进行了组织病理学分析,以确定脑样本中神经元的退化程度:结果:开阔地测试显示了明显的焦虑水平,而测试组的焦虑水平较低,但运动活动增加。生化测试表明,鱼藤酮治疗组大鼠的 AchE 水平较高,而乌头碱治疗组大鼠的 AchE 水平则显著下降。试验药物处理的大鼠也表达了较高水平的 D2 受体。经奥西美尼处理的大鼠体内α-突触核蛋白聚集减少,但在经鱼藤酮处理的大鼠脑样本中,却观察到更高的α-突触核蛋白团块:结论:乌头烯具有神经保护特性。因此,这种精油可能有助于治疗帕金森病。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The Role of Ocimene in Decreasing α-Synuclein Aggregation using Rotenone-induced Rat Model.

Background: Parkinson's disease is defined by the loss of dopaminergic neurons in the midbrain of substantia nigra associated with Lewy bodies. The precise mechanism is not yet entirely understood.

Objective: The study aims to determine whether ocimene has antiparkinsonian activity by reducing α-Synuclein aggregation levels in the brains of rotenone-induced rat models.

Methods: 36 male rats were used for six groups, with six animals in each group. Vehicle, control (rotenone, 2.5 mg/kg, i.p), standard (L-Dopa, 10 mg/kg, i.p), Test drug of low dose (66.66 mg/kg, i.p), medium dose (100 mg/kg, i.p), and high dose (200 mg/kg, i.p) were administered to the rats. The open field, actophotometer, hanging wire, and catalepsy tests were used to assess the rat's motor performance. The expressions of biomarkers such as AchE, D2 Receptor, and α- Synuclein were evaluated, and their level of expression in the brain samples was checked using ELISA. Histopathological analysis was also carried out to determine the degree of neuron degeneration in the brain samples.

Results: The open field test showed significant anxiety levels, whereas test groups showed fewer anxiety levels but increased motor activity. The biochemical tests revealed that rotenonetreated rats had higher levels of AchE, but ocimene-treated rats had a significant decrease in AchE levels. The test drug-treated rats also expressed high levels of D2 receptors. In ocimenetreated rats, α-Synuclein aggregation was reduced, however, in rotenone-treated rats' brain samples, higher clumps of α-Synuclein were observed.

Conclusion: Ocimene has neuroprotective properties. As a result, this essential oil might be helpful as a therapeutic treatment for Parkinson's disease.

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