智利家猫中巴顿氏菌 rpoB 单倍型的遗传多样性

IF 2 3区农林科学 Q4 IMMUNOLOGY

Comparative Immunology Microbiology and Infectious Diseases Pub Date : 2024-02-17 DOI:10.1016/j.cimid.2024.102150

Paulina Sepúlveda-García , Ronald Jara , Armin Mella , Gustavo Monti , Nivia Canales , Maria Eduarda Chiaradia Furquim , Marcos Rogério André , Ananda Müller

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引用次数: 0

摘要

该研究旨在确定智利猫的宿主间和宿主内巴顿菌属遗传多样性。对 79 只猫的血液 DNA 样本进行了巴顿氏菌属 rpoB 部分基因（825 bp）的 T-A 克隆，并采用 Sanger 方法进行了测序。序列被提交进行系统发育和多态性分析。成功克隆了 36 个样本（45.6%），产生了 118 个克隆，其中 109 个与鸡沙雷氏巴顿菌的一致性为 99.6%-100%，9 个与科勒氏巴顿菌的一致性为 99.8%-100%。单倍型分析得出了 29 种不同的 rpoB-B. henselae 单倍型，其中一种（hap#2）在 33 只猫中的 31 只中有较高的代表性，还有 4 种 rpoB-B. koehlerae 单倍型，其中 hap#2 在所有 3 只受 B. koehlerae 感染的猫中都有代表性。在个体猫中发现了一种以上的 rpoB -B. henselae 和 B. koehlerae 单倍型，这表明智利的猫首次同时感染了不同的 B. henselae/B. koehlerae rpoB 变体。

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Genetic diversity of Bartonella rpoB haplotypes in domestic cats from Chile

The study aimed to determine the inter and intra-host Bartonella spp. genetic diversity in cats from Chile. ‘Seventy-nine cats’ blood DNA samples qPCR Bartonella spp. positive were subjected to T-A cloning of Bartonella spp. rpoB partial gene (825 bp), and sequencing by Sanger method. The sequences were submitted to phylogenetic and polymorphism analysis. Thirty-six (45.6%) samples were successfully cloned, generating 118 clones of which 109 showed 99.6%–100% identity with Bartonella henselae whereas 9 showed 99.8–100% identity with Bartonella koehlerae. Haplotype analysis yielded 29 different rpoB-B. henselae haplotypes, one (hap#2) overrepresented in 31 out of 33 cats, and 4 rpoB-B. koehlerae haplotypes, with hap#2 represented in all 3 B. koehlerae infected cats. More than one rpoB -B. henselae and B. koehlerae haplotypes were identified in individual cats, reporting by first time coinfection by different B. henselae/B. koehlerae rpoB variants in cats from Chile.

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来源期刊

Comparative Immunology Microbiology and Infectious Diseases 医学-免疫学

CiteScore

4.60

自引率

0.00%

发文量

102

审稿时长

40 days

期刊介绍： Comparative Immunology, Microbiology & Infectious Diseases aims to respond to the concept of "One Medicine" and to provide a venue for scientific exchange. Based on the concept of "Comparative Medicine" interdisciplinary cooperation between specialists in human and animal medicine is of mutual interest and benefit. Therefore, there is need to combine the respective interest of physicians, veterinarians and other health professionals for comparative studies relevant to either human or animal medicine . The journal is open to subjects of common interest related to the immunology, immunopathology, microbiology, parasitology and epidemiology of human and animal infectious diseases, especially zoonotic infections, and animal models of human infectious diseases. The role of environmental factors in disease emergence is emphasized. CIMID is mainly focusing on applied veterinary and human medicine rather than on fundamental experimental research.