评估鱼类粘液中抗菌肽的潜在抗病毒活性。

IF 2.1 4区医学 Q3 PHARMACOLOGY & PHARMACY

Fundamental & Clinical Pharmacology Pub Date : 2024-02-20 DOI:10.1111/fcp.12996

Irmak Dik, Burak Dik, Öznur Tufan, Ayşe Er

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引用次数: 0

摘要

背景：鱼皮粘液含有先天性免疫因子，是抵御病原体的第一道物理或化学防线：本研究的主要目的是确定鲷鱼（SBr）、虹鳟鱼（RT）和鲈鱼（SBa）鱼皮粘液对单纯疱疹病毒（HSV）-1 的抗病毒活性。此外，研究还旨在将可能的抗病毒活性与抗菌肽（AMPs）联系起来，如猫肝素、肝素、galectin 2 和 C10ORF99，这些抗菌肽在粘液中的含量已被测定：方法：评估粘液对 HSV-1 病毒的抗病毒活性和氧化/抗氧化状态。此外，还分析了鱼类粘液中的 AMPs、SOD 和 CAT 活性以及免疫球蛋白 M 水平：结果：SBr、RT 和 SBa 的粘液对 HSV-1 的抗病毒活性分别为 2-4、2-5 和 2-2。与 SBa 的粘液相比，SBr 和 RT 的粘液具有更高的抗病毒活性，这可能与它们体内较高的 AMP 水平有关：结论：SBr 和 RT 的皮肤粘液可能是营养补充剂、佐剂和一种能增强抗菌剂/抗病毒剂效果的新制剂。

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Evaluation of potential antiviral activities of antimicrobial peptides in fish mucus

Background

Fish skin mucus contains innate immune factors and acts as the first line of physical or chemical defense against pathogens.

Objective

The primary aim of this study was to determine the antiviral activity of sea bream (SBr), rainbow trout (RT), and sea bass (SBa) fish skin mucus against herpes simplex virus (HSV)-1. In addition, it was aimed to associate possible antiviral activity with antimicrobial peptides (AMPs) such as cathelicidin, hepcidin, galectin 2, and C10ORF99, whose levels were determined in the mucus.

Methods

The antiviral activity and oxidative/antioxidant status of mucus against HSV-1 virus was evaluated. In addition, AMPs, SOD, and CAT activities, and immunoglobulin M levels were also analyzed in mucus of fish.

Results

Antiviral activity mucus of SBr, RT, and SBa against HSV-1 were determined as 2⁻⁴, 2⁻⁵, and 2⁻², respectively. The higher antiviral activity of SBr and RT mucus compared to the mucus of SBa can be associated with higher AMP levels in them.

Conclusion

The skin mucus of SBr and RT may be nutritional supplement, adjuvant, and a new agent that can potentiate the effects of antimicrobial/antiviral agents.

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来源期刊

Fundamental & Clinical Pharmacology 医学-药学

CiteScore

5.30

自引率

6.90%

发文量

111

审稿时长

6-12 weeks

期刊介绍： Fundamental & Clinical Pharmacology publishes reports describing important and novel developments in fundamental as well as clinical research relevant to drug therapy. Original articles, short communications and reviews are published on all aspects of experimental and clinical pharmacology including: Antimicrobial, Antiviral Agents Autonomic Pharmacology Cardiovascular Pharmacology Cellular Pharmacology Clinical Trials Endocrinopharmacology Gene Therapy Inflammation, Immunopharmacology Lipids, Atherosclerosis Liver and G-I Tract Pharmacology Metabolism, Pharmacokinetics Neuropharmacology Neuropsychopharmacology Oncopharmacology Pediatric Pharmacology Development Pharmacoeconomics Pharmacoepidemiology Pharmacogenetics, Pharmacogenomics Pharmacovigilance Pulmonary Pharmacology Receptors, Signal Transduction Renal Pharmacology Thrombosis and Hemostasis Toxicopharmacology Clinical research, including clinical studies and clinical trials, may cover disciplines such as pharmacokinetics, pharmacodynamics, pharmacovigilance, pharmacoepidemiology, pharmacogenomics and pharmacoeconomics. Basic research articles from fields such as physiology and molecular biology which contribute to an understanding of drug therapy are also welcomed.