自体移植和异种移植在矫正附着性角化牙龈缺损中的协同作用的组织学和免疫组化分析

E. A. Durnovo, E. Galkina, N. Y. Orlinskaya, M. S. Kvashnina, A. Y. Sopina
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引用次数: 0

摘要

相关性。旨在替换缺失牙齿的复杂、多阶段手术通常会导致介入区域的附着角化牙龈(AKG)不足。尽管有许多方法可用于软组织容量和美学重建,但只有少数几种方法能在术后表现出相当高的长期稳定性。因此,有必要通过对重建区域的口腔黏膜进行组织学和免疫组化检查,评估通过这种协同作用获得的组织结构。本研究分析了 57 名年龄在 28 至 59 岁之间、曾在 AKG 区进行过软组织体积和美学重建的患者的 87 份口腔黏膜活检组织。这些活检组织在软组织手术后六周采集,并进行了组织学和免疫组化检查。硬腭口腔粘膜切片作为参考样本。受检组的上皮结构显示基底层清晰,由两层细胞组成的连续结构无明显不典型性,表明细胞增殖能力很强。针对蛋白质分子的免疫组化分析表明没有炎症。Ki-67抗原的表达结果表明,细胞增殖能力得以保持,因此,在术后长达六周的远期,组织再生潜力保持在较高水平。这项研究展示了自体移植物和异种移植物结合使用的功效,这是综合组织学和免疫组化分析得出的结论。因此,这种方法是软组织容积和美学重建传统方法的一种有前途的创新替代方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Histological and immunohistochemical analysis of autografts and xenografts synergy in rectifying attached keratinized gingiva deficiency
Relevance. The complex, multi-stage surgical procedure aimed for replacing missing teeth often results in a deficiency of attached keratinized gingiva (AKG) in the intervened area. Despite numerous methods available for soft tissue volume and esthetics reconstruction, only a few demonstrate considerable long-term stability post-surgery. The quest to address this intricate issue has led to the combined utilization of autografts and xenografts Consequently, there is a need to evaluate the tissue structure obtained through this synergy via histological and immunohistochemical examination of the oral mucosa in the reconstructed region.Materials and methods. This study involved analyzing 87 biopsies of oral mucosa obtained from 57 patients aged 28 to 59 years who had undergone previous soft tissue volume and esthetics reconstruction in the AKG zone. Histological and immunohistochemical examinations were conducted on these biopsies, collected six weeks post soft tissue operation. A section of oral mucosa from the hard palate served as the reference sample.Results. The epithelial structure in the examined groups exhibited a well-defined basal layer with a continuous structure comprising two layers of cells without notable atypia, indicating a high cell proliferation capability. Immunohistochemical analysis targeting protein molecules revealed an absence of inflammation. The expression results of the Ki-67 antigen suggested the preservation of cell proliferation capacity and, consequently, the maintenance of tissue regenerative potential at a high level during the remote postoperative period, up to six weeks.Conclusion. This research showcases the efficacy of employing a combination of autografts and xenografts, a conclusion drawn from comprehensive histological and immunohistochemical analyses. Consequently, this approach presents a promising and innovative alternative to conventional methods for soft tissue volume and esthetics reconstruction.
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