伊朗人群中 TLR3 和 TLR7 基因多态性与 COVID-19 易感性的关系：一项回顾性病例对照研究

IF 1.3 Q4 MICROBIOLOGY

Iranian Journal of Microbiology Pub Date : 2024-02-12 DOI:10.18502/ijm.v16i1.14880

Masoud Parsania, Seyed Mahmood Seyed khorrami, M. Hasanzad, Negar Parsania, Sina Nagozir, N. Mokhtari, H. M. Habibabadi, A. Ghaziasadi, S. Soltani, Ali Jafarpour, Reza Pakzad, S. Jazayeri

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引用次数: 0

摘要

背景与目的：单核苷酸多态性（SNPs）等宿主基因变化是影响病毒性传染病易感性的主要因素之一。本研究旨在调查伊朗人口样本中参与免疫系统的 Toll-Like Receptor3（TLR3）和 Toll-Like Receptor7（TLR7）基因的宿主 SNP 与 COVID-19 易感性之间的关联。材料与方法：这项回顾性病例对照研究以 244 名住院的 COVID-19 患者为病例组，以 156 名症状轻微的疑似 COVID-19 患者为对照组。采用聚合酶链式反应-限制性片段长度多态性（PCR-RFLP）方法对患者的基因组 DNA 进行了 TLR7（rs179008 和 rs179009）和 TLR3（rs3775291 和 rs3775296）SNPs 测定。结果显示在病例组和对照组中发现，TLR7 基因中的 rs179008 SNP 与 COVID-19 的易感性有明显的相关性。TLR7 rs179008 A>T 多态性的 AT 基因型（杂合子）与 COVID-19 的几率增加 2.261 倍有显著相关性（P=0.003；调整 OR：2.261；99% CI：1.117-4.575）。此外，TLR7 rs179009 T>C 多态性的 TC 基因型与 COVID-19 的几率增加之间也有显著关联（P< 0.0001；调整 OR：6.818；99% CI：3.149-14.134）。TLR3 rs3775291 和 rs3775296 基因型的多态性频率在病例组和对照组之间无显著差异（P> 0.004167）。结论TLR7 rs179008 和 rs179009 基因型中的 SNPs 被认为是可能影响 COVID-19 易感性的宿主遗传因素。在伊朗人群中，TLR3 的 SNPs（rs3775296 和 rs3775291）与 COVID-19 没有显著关联。

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Association of polymorphisms in TLR3 and TLR7 genes with susceptibility to COVID-19 among Iranian population: a retrospective case-control study

Background and Objectives: Host genetic changes like single nucleotide polymorphisms (SNPs) are one of the main fac- tors influencing susceptibility to viral infectious diseases. This study aimed to investigate the association between the host SNP of Toll-Like Receptor3 (TLR3) and Toll-Like Receptor7 (TLR7) genes involved in the immune system and susceptibil- ity to COVID-19 in a sample of the Iranian population. Materials and Methods: This retrospective case-control study evaluated 244 hospitalized COVID-19 patients as the case group and 156 suspected COVID-19 patients with mild signs as the control group. The genomic DNA of patients was gen- otyped for TLR7 (rs179008 and rs179009) and TLR3 (rs3775291 and rs3775296) SNPs using the polymerase chain reac- tion-restriction fragment length polymorphism (PCR-RFLP) method. Results: A significant association between rs179008 SNP in the TLR7 gene and the susceptibility of COVID-19 was found between case and control groups. The AT genotype (Heterozygous) of TLR7 rs179008 A>T polymorphism showed a sig- nificant association with a 2.261-fold increased odds of COVID-19 (P=0.003; adjusted OR: 2.261; 99% CI: 1.117-4.575). In addition, a significant association between TC genotype of TLR7 rs179009 T>C polymorphism and increased odds of COVID-19 (P< 0.0001; adjusted OR: 6.818; 99% CI: 3.149-14.134) were determined. The polymorphism frequency of TLR3 rs3775291 and rs3775296 genotypes were not significantly different between the case and control groups (P> 0.004167). Conclusion: SNPs in TLR7 rs179008 and rs179009 genotypes are considered host genetic factors that could be influenced individual susceptibility to COVID-19. The SNPs in TLR3 (rs3775296 and rs3775291) showed no significant association with COVID-19 in Iranian population.

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来源期刊

Iranian Journal of Microbiology MICROBIOLOGY-

CiteScore

2.40

自引率

7.10%

发文量

审稿时长

12 weeks

期刊介绍： The Iranian Journal of Microbiology (IJM) is an international, multi-disciplinary, peer-reviewed journal that provides rapid publication of the most advanced scientific research in the areas of basic and applied research on bacteria and other micro-organisms, including bacteria, viruses, yeasts, fungi, microalgae, and protozoa concerning the development of tools for diagnosis and disease control, epidemiology, antimicrobial agents, clinical microbiology, immunology, Genetics, Genomics and Molecular Biology. Contributions may be in the form of original research papers, review articles, short communications, case reports, technical reports, and letters to the Editor. Research findings must be novel and the original data must be available for review by the Editors, if necessary. Studies that are preliminary, of weak originality or merely descriptive as well as negative results are not appropriate for the journal. Papers considered for publication must be unpublished work (except in an abstract form) that is not under consideration for publication anywhere else, and all co-authors should have agreed to the submission. Manuscripts should be written in English.