检测结核分枝杆菌的 S 基因型并澄清欧美血统中有争议的 Spoligotypes 的位置

IF 1.3 4区生物学 Q4 MICROBIOLOGY

Microbiology Pub Date : 2024-02-07 DOI:10.1134/s0026261723603457

I. V. Mokrousov, S. N. Zhdanova, V. Valcheva, G. I. Alekseeva, M. K. Vinokurova, N. E. Evdokimova, O. B. Ogarkov

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引用次数: 0

摘要

摘要--S 基因型（首次描述于西西里岛，因此得名）是欧美结核分枝杆菌系的一部分。在此，我们报告了一种基于 Rv0557 基因 PCR-RFLP 的检测方法。该方法在已知全基因组序列的菌株上进行了优化，并进一步应用于筛选来自俄罗斯、保加利亚、中国、日本和越南的 DNA 收集物。PCR-RFLP 分析证实了具有 Spoligotypes SIT34（S 的祖先）和 SIT1253（雅库特菌株）的菌株的 S 基因型。来自保加利亚的菌株具有 SIT4 和 SIT125 的节略 spoligoprofiles，也被归入 S 基因型。所开发的方法可快速检测结核杆菌的 S 基因型，并可靠地解决有争议的 spoligotypes 系统发育模糊问题。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Detection of the Mycobacterium tuberculosis S-Genotype and Clarification of the Position of Controversial Spoligotypes of the Euro-American Lineage

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Detection of the Mycobacterium tuberculosis S-Genotype and Clarification of the Position of Controversial Spoligotypes of the Euro-American Lineage

Abstract—

The S genotype (first described in Sicily, hence the name) is a part of the Euro-American lineage of Mycobacterium tuberculosis. Here, we report a method for its detection based on PCR-RFLP of the Rv0557 gene. The method was optimized on strains with known whole genome sequences and further applied to screening the DNA collections from Russia, Bulgaria, China, Japan, and Vietnam. PCR-RFLP analysis confirmed the S genotype in strains with the spoligotypes SIT34 (ancestral to S) and SIT1253 (Yakutia strains). Strains from Bulgaria with abridged spoligoprofiles SIT4 and SIT125 were also assigned to the S genotype. The developed method provides for rapid detection of the M. tuberculosis S genotype and reliably resolves phylogenetic ambiguity of the disputed spoligotypes.

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来源期刊

Microbiology 生物-微生物学

CiteScore

2.40

自引率

13.30%

发文量

审稿时长

6-12 weeks

期刊介绍： Microbiology is an is an international peer reviewed journal that covers a wide range of problems in the areas of fundamental and applied microbiology. The journal publishes experimental and theoretical papers, reviews on modern trends in different fields of microbiological science, and short communications with descriptions of unusual observations. The journal welcomes manuscripts from all countries in the English or Russian language.