Xian Wu, Liying Sun, Lei Huang, Chenwei Huang, Kuixia Sun, Haixia Li
{"title":"与白色念珠菌菌丝诱导的单核细胞胞外陷阱的形成有关的蛋白质组变化。","authors":"Xian Wu, Liying Sun, Lei Huang, Chenwei Huang, Kuixia Sun, Haixia Li","doi":"10.2217/fmb-2023-0029","DOIUrl":null,"url":null,"abstract":"<p><p><b>Background:</b> The interaction between the host and <i>Candida albicans</i> is dynamic and intricate. We performed proteomic analysis to explore monocyte<i>-C. albicans</i> hyphae interaction. <b>Materials & methods:</b> Primary human monocytes were stimulated by heat-killed <i>C. albicans</i> hyphae and their proteins were profiled by tandem liquid chromatography with mass spectrometry (LC-MS/MS). <b>Results:</b> Based on the protein database of different species for analysis, we found that stimulation of monocytes by hyphae was accompanied by upregulation of histones and activation of extracellular traps (ETs) formation pathway. Meanwhile, monocyte ETs (MoETs) were evoked by synthesis or alteration of <i>C. albicans</i> cell wall proteins expression during the morphological switch to hyphal. <b>Conclusion:</b> MoETs formation is linked to cell wall proteins of <i>C. albicans</i> hyphae.</p>","PeriodicalId":12773,"journal":{"name":"Future microbiology","volume":" ","pages":"307-316"},"PeriodicalIF":2.5000,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Proteomic alterations associated with the formation of monocyte extracellular trap induced by <i>Candida albicans</i> hyphae.\",\"authors\":\"Xian Wu, Liying Sun, Lei Huang, Chenwei Huang, Kuixia Sun, Haixia Li\",\"doi\":\"10.2217/fmb-2023-0029\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p><b>Background:</b> The interaction between the host and <i>Candida albicans</i> is dynamic and intricate. We performed proteomic analysis to explore monocyte<i>-C. albicans</i> hyphae interaction. <b>Materials & methods:</b> Primary human monocytes were stimulated by heat-killed <i>C. albicans</i> hyphae and their proteins were profiled by tandem liquid chromatography with mass spectrometry (LC-MS/MS). <b>Results:</b> Based on the protein database of different species for analysis, we found that stimulation of monocytes by hyphae was accompanied by upregulation of histones and activation of extracellular traps (ETs) formation pathway. Meanwhile, monocyte ETs (MoETs) were evoked by synthesis or alteration of <i>C. albicans</i> cell wall proteins expression during the morphological switch to hyphal. <b>Conclusion:</b> MoETs formation is linked to cell wall proteins of <i>C. albicans</i> hyphae.</p>\",\"PeriodicalId\":12773,\"journal\":{\"name\":\"Future microbiology\",\"volume\":\" \",\"pages\":\"307-316\"},\"PeriodicalIF\":2.5000,\"publicationDate\":\"2024-03-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Future microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.2217/fmb-2023-0029\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/2/15 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q3\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Future microbiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.2217/fmb-2023-0029","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/2/15 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
Proteomic alterations associated with the formation of monocyte extracellular trap induced by Candida albicans hyphae.
Background: The interaction between the host and Candida albicans is dynamic and intricate. We performed proteomic analysis to explore monocyte-C. albicans hyphae interaction. Materials & methods: Primary human monocytes were stimulated by heat-killed C. albicans hyphae and their proteins were profiled by tandem liquid chromatography with mass spectrometry (LC-MS/MS). Results: Based on the protein database of different species for analysis, we found that stimulation of monocytes by hyphae was accompanied by upregulation of histones and activation of extracellular traps (ETs) formation pathway. Meanwhile, monocyte ETs (MoETs) were evoked by synthesis or alteration of C. albicans cell wall proteins expression during the morphological switch to hyphal. Conclusion: MoETs formation is linked to cell wall proteins of C. albicans hyphae.
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Future Microbiology delivers essential information in concise, at-a-glance article formats. Key advances in the field are reported and analyzed by international experts, providing an authoritative but accessible forum for this increasingly important and vast area of research.
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