伊朗西南部胡齐斯坦省血液透析患者的弓形虫分子检测和系统发育分析。

IF 3.6 Q1 TROPICAL MEDICINE
Saba Yarahmadi, Jasem Saki, Reza Arjmand, Heshmatolah Shahbazian
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引用次数: 0

摘要

背景:弓形虫(T. gondii)感染的诊断和基因特征描述可对预防弓形虫病的危险后果产生重大影响,尤其是在免疫力低下的人群中:本次调查的目的是评估血液透析患者血液样本中冈地弓形虫的频率和基因分型:在本次调查中,共采集了 379 份血液样本,这些样本来自伊朗西南部阿瓦士教学医院的血液透析患者。样本采用针对 B1 基因的巢式 PCR 方法进行评估,然后进行测序并构建系统发生树:结果:通过 Nested PCR,在 112 份(29.55%)血液样本中发现了淋球菌 DNA。淋病双球菌的扩增子与 GenBank 序列具有很高的一致性。系统进化分析表明,所有序列都与淋病双球菌的 I 型密切相关:由于血液透析患者中I型弓形虫的发病率较高,我们建议对弓形虫进行系统筛查,以监测血液透析期间弓形虫可能的传播。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Toxoplasma gondii molecular detection and phylogenetic analysis in hemodialysis patients from Khuzestan, Southwest Iran.

Background: The diagnosis and genetic characterization of Toxoplasma gondii (T. gondii) infection can make a significant influence to the prevention of the dangerous consequences of toxoplasmosis, particularly in immunocompromised people.

Objective: The aim of this investigation was to assess the frequency and genotyping of T. gondii in blood samples of patients with hemodialysis.

Materials and methods: In the current investigation, a total of 379 blood samples were taken from subjects with hemodialysis who were referred to teaching hospital of Ahvaz in the southwest of Iran. The samples were evaluated using the Nested PCR by targeting the B1 gene, and then, sequencing and phylogenetic tree were constructed.

Results: T. gondii DNA was found in 112 (29.55%) of the blood samples by Nested PCR. Amplicons from T. gondii revealed high identity with GenBank sequences. The phylogenetic analysis revealed that all sequences were closely related to Type I of T. gondii.

Conclusion: Because of the high incidence of toxoplasmosis with type I prevalent in hemodialysis patients, we recommend a systematic screening for toxoplasmosis to carry out for monitoring the possible dissemination of toxoplasmosis during hemodialysis.

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来源期刊
Tropical Medicine and Health
Tropical Medicine and Health TROPICAL MEDICINE-
CiteScore
7.00
自引率
2.20%
发文量
90
审稿时长
11 weeks
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