{"title":"青鳉收费样受体基因家族昼夜节律表达的综合分析","authors":"Takahiko Hata , Hidetoshi Shimawaki , Suzuka Setoguchi , Natsuki Morimoto , Jun-ichi Hikima , Masahiro Sakai , Tomoya Kono","doi":"10.1016/j.dci.2024.105143","DOIUrl":null,"url":null,"abstract":"<div><p>Several immune-related genes, including Toll-like receptors (TLR), are associated with circadian rhythms in mammals. However, information on the circadian rhythmic expression of TLRs in fish is limited. In this study, we aimed to analyze the regulation of diel oscillations in the expression of TLR genes in Japanese medaka (<em>Oryzias latipes</em>). The expression analysis revealed diel expression patterns of <em>tlr1</em>, <em>tlr5m</em>, <em>tlr21</em>, and clock genes (<em>bmal1</em> and <em>clock1</em>) under a 12 h light:12 h dark cycle. The clock gene response element (E-box) was identified in the transcriptional regulatory regions of <em>tlr1</em>, <em>tlr5m</em>, and <em>tlr21</em>. Moreover, overexpressed <em>bmal1</em> and <em>clock1</em> enhanced expression levels of <em>tlr1</em>, <em>tlr5m</em>, and <em>tlr21</em> in medaka embryo (OLHdrR-e3) cells. The expression of <em>tlr1</em>, <em>tlr5m</em>, and <em>tlr21</em> was significantly decreased in OLHdrR-e3 after generating a <em>bmal1</em> knockdown using a morpholino oligo. These results indicate the regulation of the diel rhythmic expression of several fish TLRs by clock genes.</p></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":null,"pages":null},"PeriodicalIF":2.7000,"publicationDate":"2024-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Comprehensive analysis of diel rhythmic expression of the medaka toll-like receptor gene family\",\"authors\":\"Takahiko Hata , Hidetoshi Shimawaki , Suzuka Setoguchi , Natsuki Morimoto , Jun-ichi Hikima , Masahiro Sakai , Tomoya Kono\",\"doi\":\"10.1016/j.dci.2024.105143\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Several immune-related genes, including Toll-like receptors (TLR), are associated with circadian rhythms in mammals. However, information on the circadian rhythmic expression of TLRs in fish is limited. In this study, we aimed to analyze the regulation of diel oscillations in the expression of TLR genes in Japanese medaka (<em>Oryzias latipes</em>). The expression analysis revealed diel expression patterns of <em>tlr1</em>, <em>tlr5m</em>, <em>tlr21</em>, and clock genes (<em>bmal1</em> and <em>clock1</em>) under a 12 h light:12 h dark cycle. The clock gene response element (E-box) was identified in the transcriptional regulatory regions of <em>tlr1</em>, <em>tlr5m</em>, and <em>tlr21</em>. Moreover, overexpressed <em>bmal1</em> and <em>clock1</em> enhanced expression levels of <em>tlr1</em>, <em>tlr5m</em>, and <em>tlr21</em> in medaka embryo (OLHdrR-e3) cells. The expression of <em>tlr1</em>, <em>tlr5m</em>, and <em>tlr21</em> was significantly decreased in OLHdrR-e3 after generating a <em>bmal1</em> knockdown using a morpholino oligo. These results indicate the regulation of the diel rhythmic expression of several fish TLRs by clock genes.</p></div>\",\"PeriodicalId\":11228,\"journal\":{\"name\":\"Developmental and comparative immunology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.7000,\"publicationDate\":\"2024-02-09\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Developmental and comparative immunology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0145305X24000156\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"FISHERIES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Developmental and comparative immunology","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0145305X24000156","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"FISHERIES","Score":null,"Total":0}
Comprehensive analysis of diel rhythmic expression of the medaka toll-like receptor gene family
Several immune-related genes, including Toll-like receptors (TLR), are associated with circadian rhythms in mammals. However, information on the circadian rhythmic expression of TLRs in fish is limited. In this study, we aimed to analyze the regulation of diel oscillations in the expression of TLR genes in Japanese medaka (Oryzias latipes). The expression analysis revealed diel expression patterns of tlr1, tlr5m, tlr21, and clock genes (bmal1 and clock1) under a 12 h light:12 h dark cycle. The clock gene response element (E-box) was identified in the transcriptional regulatory regions of tlr1, tlr5m, and tlr21. Moreover, overexpressed bmal1 and clock1 enhanced expression levels of tlr1, tlr5m, and tlr21 in medaka embryo (OLHdrR-e3) cells. The expression of tlr1, tlr5m, and tlr21 was significantly decreased in OLHdrR-e3 after generating a bmal1 knockdown using a morpholino oligo. These results indicate the regulation of the diel rhythmic expression of several fish TLRs by clock genes.
期刊介绍:
Developmental and Comparative Immunology (DCI) is an international journal that publishes articles describing original research in all areas of immunology, including comparative aspects of immunity and the evolution and development of the immune system. Manuscripts describing studies of immune systems in both vertebrates and invertebrates are welcome. All levels of immunological investigations are appropriate: organismal, cellular, biochemical and molecular genetics, extending to such fields as aging of the immune system, interaction between the immune and neuroendocrine system and intestinal immunity.