艰难梭菌的显微镜检查方法。

IF 2.5 3区生物学 Q3 MICROBIOLOGY

Anaerobe Pub Date : 2024-02-08 DOI:10.1016/j.anaerobe.2024.102822

Chenlin Hu, Kevin W. Garey

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引用次数: 0

摘要

包括光和荧光、透射电子显微镜（TEM）、扫描电子显微镜（SEM）和低温电子显微镜在内的显微镜技术已被广泛用于从分子、细胞、群落和结构生物学层面观察艰难梭菌。本综述总结了用于研究艰难梭菌生命周期和毒力（孢子、发芽）或应用（艰难梭菌检测或抗菌药物的使用）不同方面的显微镜工具（荧光和报告系统）。有了这些不断发展的技术，显微镜工具将能够找到更广泛的应用，并解决研究艰难梭菌和艰难梭菌感染的更具挑战性的问题。

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Microscopy methods for Clostridioides difficile

Microscopic technologies including light and fluorescent, transmission electron microscopy (TEM), scanning electron microscopy (SEM), and cryo-electron microscopy have been widely utilized to visualize Clostridioides difficile at the molecular, cellular, community, and structural biology level. This comprehensive review summarizes the microscopy tools (fluorescent and reporter system) in their use to study different aspects of C. difficile life cycle and virulence (sporulation, germination) or applications (detection of C. difficile or use of antimicrobials). With these developing techniques, microscopy tools will be able to find broader applications and address more challenging questions to study C. difficile and C. difficile infection.

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来源期刊

Anaerobe 生物-微生物学

CiteScore

5.20

自引率

8.70%

发文量

137

审稿时长

76 days

期刊介绍： Anaerobe is essential reading for those who wish to remain at the forefront of discoveries relating to life processes of strictly anaerobes. The journal is multi-disciplinary, and provides a unique forum for those investigating anaerobic organisms that cause infections in humans and animals, as well as anaerobes that play roles in microbiomes or environmental processes. Anaerobe publishes reviews, mini reviews, original research articles, notes and case reports. Relevant topics fall into the broad categories of anaerobes in human and animal diseases, anaerobes in the microbiome, anaerobes in the environment, diagnosis of anaerobes in clinical microbiology laboratories, molecular biology, genetics, pathogenesis, toxins and antibiotic susceptibility of anaerobic bacteria.