移液管奥运会:本科生实验室培训的一项有吸引力的练习。

Troy A Richter, Cooper D Wilkinson, Brian B Griffiths, Richard G Hunter
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引用次数: 0

摘要

移液是几乎所有分子神经科学方法中都要用到的重要技术,包括但不限于 PCR、反转录、免疫组化、染色质免疫沉淀和细胞培养。COVID-19 大流行剥夺了本科生亲自练习实验室技术的时间。为此,我们设计了一种标准化、快速而有趣的方法,指导学生掌握移液、系列稀释和基本统计分析的基本知识。在此,我们提供了一个标准化的方案,供教师用于向本科生传授宝贵的技能,同时提供友好的竞争。我们还提供了一个本科生执行该方案步骤的示例,并附有示例结果和三个本科生前两次尝试的结果。这项练习为实验室提供了一种方法,让本科生重新学习实验室基础知识,同时使培训标准化,从而节省了因大流行病而损失的时间。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Pipette Olympics: An Engaging Exercise for Undergraduate Laboratory Training.

Pipetting is an important technique used in almost every molecular neuroscience method including but not limited to, PCR, reverse transcription, immunohistochemistry, chromatin immunoprecipitation, and cell culture. The COVID-19 pandemic has robbed the undergraduate population of time to practice in person laboratory techniques. In response, we have devised a standardized, quick, and fun way to instruct students on the fundamentals of pipetting, serial dilutions, and basic statistical analysis. Here, we offer a standardized protocol for instructors to use to teach undergraduates valuable skills while providing friendly competition. We also offer an example of an undergraduate performing the steps of this protocol with example results and the results from three separate undergrads' first two attempts. This exercise provides laboratories with a method to reintroduce undergraduates to lab basics while standardizing the training thereby saving time lost to the pandemic.

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