利用 MRM/PRM 方法靶向分析过甲基化 N-聚糖

Q4 Biochemistry, Genetics and Molecular Biology

Methods in molecular biology Pub Date : 2024-01-01 DOI:10.1007/978-1-0716-3666-4_15

Cristian D Gutierrez Reyes, Akeem Sanni, Damir Mogut, Moyinoluwa Adeniyi, Parisa Ahmadi, Mojgan Atashi, Sherifdeen Onigbinde, Yehia Mechref

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引用次数: 0

摘要

靶向质谱分析因其高灵敏度和高精确度而被广泛应用于各种海洋组学领域，作为一种验证策略。这种方法已成功用于蛋白质、糖类、脂类和代谢物的结构分析。多重反应监测（MRM）和平行反应监测（PRM）一直是生物大分子定向结构研究的首选方法。这些靶向分析简化了分析工作流程，减少了背景干扰，提高了选择性/特异性，从而可以对复杂生物基质中的过甲基化 N-聚糖进行可靠的定量分析。

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Targeted Analysis of Permethylated N-Glycans Using MRM/PRM Approaches.

Targeted mass spectrometric analysis is widely employed across various omics fields as a validation strategy due to its high sensitivity and accuracy. The approach has been successfully employed for the structural analysis of proteins, glycans, lipids, and metabolites. Multiple reaction monitoring (MRM) and parallel reaction monitoring (PRM) have been the methods of choice for targeted structural studies of biomolecules. These target analyses simplify the analytical workflow, reduce background interference, and increase selectivity/specificity, allowing for a reliable quantification of permethylated N-glycans in complex biological matrices.

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来源期刊

Methods in molecular biology Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

2.00

自引率

0.00%

发文量

3536

期刊介绍： For over 20 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by-step fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice.