低温技术。

IF 1 4区 生物学 Q3 BIOLOGY
Cryo letters Pub Date : 2023-11-01
J Hagedorn, G Alkurdi, S-A Barker, R Brunotte, T Deeb, O Hubenia, D Khayyat, S Leal-Marin, T Rittinghaus, B Glasmacher
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引用次数: 0

摘要

在极低温度下冷冻生物材料的过程被称为低温保存。为了确保长期保存细胞和组织,需要使用低温,因为在低温条件下,生物过程(包括生化过程)会停止,因此可以保持其结构和功能的完整性。低温保存领域在 20 世纪变得更加突出,并成为细胞治疗、组织工程或辅助受精等不同应用中不可避免的技术。在这项工作中,我们概述了低温技术领域有关活细胞冷冻、储存和解冻的各种技术。第一部分涉及冷冻过程,首先介绍冷冻保护剂的保护机制和成分,然后介绍冷冻成像、微流控系统以及目前可用的冷冻和生物保存设备。第二部分重点介绍解冻过程以及用于短期储存生物材料和构建体的低温保存。Doi.org/10.54680/fr23610110112.
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The technology in cryotechnology.

The process of freezing biological material at extremely low temperatures is known as cryopreservation. To ensure the preservation of cells and tissues over an extended period of time, low temperatures are applied since biological processes, including the biochemical ones, come to a halt under cryogenic conditions and thus it is possible to maintain their structural and functional integrity. The field of cryopreservation gained more prominence in the 20th century and emerged as an unavoidable technology for different applications such as cell therapy, tissue engineering, or assisted fertilization. In this work we provide an overview of various technologies in the field of cryotechnology with regard to the freezing, storage and thawing of living cells. The first part covers the freezing process, starting with cryoprotective agents regarding their protection mechanisms and compositions, passing by cryo-imaging, micro-fluidic systems, and the currently available freezing and biobanking equipment. The second part focusses on the thawing process as well as the hypothermic preservation for the short-term storage of biological materials and constructs. Doi.org/10.54680/fr23610110112.

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来源期刊
Cryo letters
Cryo letters 生物-生理学
CiteScore
1.80
自引率
10.00%
发文量
50
审稿时长
1 months
期刊介绍: A bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.
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