评估线虫秀丽隐杆线虫的多谷氨酰胺束聚集。

4区生物学 Q4 Biochemistry, Genetics and Molecular Biology

Methods in cell biology Pub Date : 2024-01-01 Epub Date: 2022-10-14 DOI:10.1016/bs.mcb.2022.09.003

Aggeliki Sotiriou, Christina Ploumi, Nikolaos Charmpilas, Nektarios Tavernarakis

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引用次数: 0

摘要

蛋白质组的完整性是细胞功能和生物活力的先决条件。蛋白质组的完整性受到破坏被认为是衰老过程的固有组成部分，并且与年龄相关的神经退行性病变的发生有关。尽管蛋白质稳态（proteostasis）的分子基础已被广泛研究，但其调控的几个方面仍然难以捉摸。线虫秀丽隐杆线虫已成为研究体内易聚集人类蛋白质动态的多功能异源模式生物。在这里，我们描述了一种分析多聚谷氨酰胺（polyQ）束聚集的实验方法，它是衰老过程中蛋白质稳态状态的一种衡量标准。

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Assessing polyglutamine tract aggregation in the nematode Caenorhabditis elegans.

Proteome integrity is a prerequisite for cellular functionality and organismal viability. Its compromise is considered an inherent part of the aging process and has been associated with the onset of age-related, neurodegenerative pathologies. Although the molecular underpinnings of protein homeostasis (proteostasis) have been extensively studied, several aspects of its regulation remain elusive. The nematode Caenorhabditis elegans has emerged as a versatile, heterologous model organism to study the dynamics of aggregation-prone human proteins in vivo. Here, we describe an experimental pipeline for the analysis of polyglutamine (polyQ) tract aggregation, as a measure of the state of proteostasis, during aging.

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来源期刊

Methods in cell biology 生物-细胞生物学

CiteScore

3.10

自引率

0.00%

发文量

125

审稿时长

3 months

期刊介绍： For over fifty years, Methods in Cell Biology has helped researchers answer the question "What method should I use to study this cell biology problem?" Edited by leaders in the field, each thematic volume provides proven, state-of-art techniques, along with relevant historical background and theory, to aid researchers in efficient design and effective implementation of experimental methodologies. Over its many years of publication, Methods in Cell Biology has built up a deep library of biological methods to study model developmental organisms, organelles and cell systems, as well as comprehensive coverage of microscopy and other analytical approaches.