Ingrid Figueroa-Galvis , Orson Mestanza , Andrea Muñoz , Victor A. Ramos-Duarte , Javier Vanegas
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引用次数: 0
摘要
高盐度会抑制氮循环,而氮循环对沿海红树林生态系统的生物地球化学变化至关重要。我们研究了盐度梯度(电导率为 5.27 mS cm-1-38.64 mS cm-1)下的根瘤藻土壤,以了解高盐度如何影响细菌群落和氮代谢活动。16S rRNA 基因的扩增子测序研究了细菌种群概况,而全枪元基因组测序则评估了功能基因潜力。芽孢杆菌、脱硫单胞菌、甲基海洋杆菌和硝化细菌在属中占主导地位,而蛋白杆菌、放线菌和类杆菌在门中占主导地位。在高盐度条件下,硝化细菌占优势。高盐度土壤抑制了氮循环基因的丰度:nifH、nxrAB、nirS、nirK、norB、nirB 和 nirA。在通过扩增子测序发现的硝化菌群体中,氨氧化细菌(如亚硝化球菌和亚硝化单胞菌)随着盐度的增加而减少。在高盐度条件下,亚硝酸盐氧化细菌(如亚硝酸螺菌和亚硝酸单胞菌)数量增加,而亚硝酸球菌和亚硝酸单胞菌数量减少。盐度降低了大多数硝化细菌群落成员的氮基因丰度,抑制了氮循环。
High salinity suppresses nitrogen cycle genes and shifts nitrifier communities in the black mangrove rhizosphere
High salinity inhibits the nitrogen cycle, which is crucial to biogeochemical changes in coastal mangrove ecosystems. We examined Avicennia germinans rhizosphere soil over a salinity gradient (electrical conductivities of 5.27 mS cm−1–38.64 mS cm−1) to see how high salinity affects the bacterial community and metabolic nitrogen activities. Amplicon sequencing of the 16S rRNA gene examined the bacterial population profile, whereas full shotgun metagenome sequencing assessed functional genetic potential. Bacillus, Desulfuromonas, Methyloceanibacter, and Nitrospira dominated the genera, whereas Proteobacteria, Actinobacteria, and Bacteroidetes dominated the phyla. Nitrospirae dominated at high salinity. High soil salinity suppressed nitrogen cycle gene abundances: nifH, nxrAB, nirS, nirK, norB, nirB, and nirA. Ammonia-oxidizing bacteria like Nitrosococcus and Nitrosomonas decreased with salinity in the nitrifier population discovered by amplicon sequencing. Nitrite-oxidizing bacteria like Nitrospira and Nitrospina rose at high salinity, whereas Nitrococcus and Nitrolancea declined. Salinity reduces nitrogen gene abundances in most nitrifier community members, inhibiting the nitrogen cycle.
RhizosphereAgricultural and Biological Sciences-Agronomy and Crop Science
CiteScore
5.70
自引率
8.10%
发文量
155
审稿时长
29 days
期刊介绍:
Rhizosphere aims to advance the frontier of our understanding of plant-soil interactions. Rhizosphere is a multidisciplinary journal that publishes research on the interactions between plant roots, soil organisms, nutrients, and water. Except carbon fixation by photosynthesis, plants obtain all other elements primarily from soil through roots.
We are beginning to understand how communications at the rhizosphere, with soil organisms and other plant species, affect root exudates and nutrient uptake. This rapidly evolving subject utilizes molecular biology and genomic tools, food web or community structure manipulations, high performance liquid chromatography, isotopic analysis, diverse spectroscopic analytics, tomography and other microscopy, complex statistical and modeling tools.