Beata Irena Cejko , Dorota Fopp-Bayat , Roman Kujawa
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It was noted that the addition of the lowest concentration (0.1%) of albumin, casein, or the pluronic to BS had a significant effect on the motility and kinetic parameters of whitefish sperm compared to pure BS. BS supplemented with 0.2–0.5% albumin was the most appropriate variant used for whitefish sperm motility activation in the present experiment. BS supplemented with the pluronic at 1.0–2.0% concentrations resulted in significantly higher values of almost all CASA parameters compared to casein at the same concentrations. Moreover, CASA parameters determined in this variant of the pluronic (1.0–2.0%) were similar to those when BS was supplemented with the same albumin concentrations. 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引用次数: 0
摘要
精子粘附在玻璃载玻片上是使用 CASA 系统分析鱼类精子活力的主要问题之一。为了缓解这一问题,白蛋白是最常添加到活化溶液中的补充剂。然而,在欧洲白鲑(Coregonus lavaretus)精子活力分析中,除白蛋白(不同浓度)外,尚无使用其他补充剂的数据。本研究使用三种抗粘附补充剂(白蛋白、酪蛋白、Pluronic F-127)对这一问题进行了调查,这些补充剂以不同浓度(0.0%;0.1%;0.2%;0.5%;1.0%;2.0%)添加到比拉德溶液(BS:20 mM Tris、1 mM CaCl2、154 mM NaCl、30 mM glycine,pH 9.0)中。结果表明,与纯 BS 相比,在 BS 中添加最低浓度(0.1%)的白蛋白、酪蛋白或 pluronic 对白鲑精子的活力和动力学参数有显著影响。在本实验中,添加了 0.2-0.5% 白蛋白的 BS 是最适合用于激活白鲑精子活力的变体。与相同浓度的酪蛋白相比,添加了 1.0%-2.0% pluronic 的 BS 可显著提高几乎所有 CASA 参数值。此外,用这种变体 pluronic(1.0-2.0%)测定的 CASA 参数与用相同浓度的白蛋白补充 BS 时的 CASA 参数相似。这表明,可以用 BS 中较高浓度的质子代替白蛋白来分析白鲑精子的活力。
Effect of anti-adhesive supplement doses on sperm motility characteristics in the European whitefish (Coregonus lavaretus)
Sperm adhering to glass slides is one of the main problems during fish sperm motility analyses with CASA systems. To mitigate this, albumin is the supplement added most frequently to activating solutions. However, there is no data on the use of supplements other than albumin (in various concentrations) in analyses of European whitefish (Coregonus lavaretus) sperm motility. This issue was investigated in the presented research using three anti-adhesive supplements (albumin, casein, Pluronic F-127) that were added to Billard solution (BS: 20 mM Tris, 1 mM CaCl2, 154 mM NaCl, 30 mM glycine at pH 9.0) at different concentrations (0.0; 0.1; 0.2; 0.5; 1.0; 2.0%). It was noted that the addition of the lowest concentration (0.1%) of albumin, casein, or the pluronic to BS had a significant effect on the motility and kinetic parameters of whitefish sperm compared to pure BS. BS supplemented with 0.2–0.5% albumin was the most appropriate variant used for whitefish sperm motility activation in the present experiment. BS supplemented with the pluronic at 1.0–2.0% concentrations resulted in significantly higher values of almost all CASA parameters compared to casein at the same concentrations. Moreover, CASA parameters determined in this variant of the pluronic (1.0–2.0%) were similar to those when BS was supplemented with the same albumin concentrations. This indicated that instead of albumin, the pluronic at higher concentrations in BS might be used to analyze whitefish sperm motility.
期刊介绍:
Animal Reproduction Science publishes results from studies relating to reproduction and fertility in animals. This includes both fundamental research and applied studies, including management practices that increase our understanding of the biology and manipulation of reproduction. Manuscripts should go into depth in the mechanisms involved in the research reported, rather than a give a mere description of findings. The focus is on animals that are useful to humans including food- and fibre-producing; companion/recreational; captive; and endangered species including zoo animals, but excluding laboratory animals unless the results of the study provide new information that impacts the basic understanding of the biology or manipulation of reproduction.
The journal''s scope includes the study of reproductive physiology and endocrinology, reproductive cycles, natural and artificial control of reproduction, preservation and use of gametes and embryos, pregnancy and parturition, infertility and sterility, diagnostic and therapeutic techniques.
The Editorial Board of Animal Reproduction Science has decided not to publish papers in which there is an exclusive examination of the in vitro development of oocytes and embryos; however, there will be consideration of papers that include in vitro studies where the source of the oocytes and/or development of the embryos beyond the blastocyst stage is part of the experimental design.