Tridax procumbens L. Extracts:民族药用伤口愈合研究

IF 0.1 Q4 MEDICINE, RESEARCH & EXPERIMENTAL
Hemashenpagam N, Selvajeyanthi S, Theras G Abishma, Bavin Kumar V, Charan S, Boopathi S, Mithun S K
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引用次数: 0

摘要

本研究旨在评估从三七(一种部落社区传统用于治疗伤口的植物)中提取的各种提取物(甲醇、乙醇、正己烷、氯仿和水溶液)的伤口愈合活性和抗菌潜力。研究包括使用琼脂井扩散法评估提取物的抗菌活性,以及制备注入植物提取物的纱布和软膏。使用索氏提取器提取植物化合物时使用了不同的溶剂。针对从感染伤口样本中分离出来的微生物,在不同培养基(营养琼脂、西曲肽琼脂、血琼脂、麦康凯琼脂和 MRSA)上培养后,测试了提取物的抗菌活性。使用琼脂井扩散法测定最小抑菌浓度,以确定植物提取物的抗菌功效。在从受感染的伤口样本中分离出的其他试验生物(链球菌、假单胞菌、变形杆菌)中,踯躅草乙醇提取物对金黄色葡萄球菌和大肠杆菌的抗菌活性最高。进行了植物化学测试,以确定促进伤口愈合的生物活性化合物,随后用柱色谱法分离了这些化合物,并通过薄层色谱法进行了纯化。傅立叶变换红外光谱分析确定了存在的官能团,并进行了高效液相色谱进一步表征。经鉴定,植物提取物中的黄酮类、单宁酸和萜类化合物是促进伤口愈合的活性成分。此外,还制备了浸泡了植物提取物的纱布,以促进伤口愈合,并使用刺五加的乙醇提取物和软膏基质配制了软膏。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Tridax procumbens L. Extracts: Ethnomedicinal Wound Healing Investigation
This research aims to assess the wound healing activity and antimicrobial potential of various extracts (Methanol, Ethanol, n-Hexane, Chloroform, and Aqueous) derived from Tridax procumbens, a plant traditionally used in wound treatment by tribal communities. The study includes evaluating the antimicrobial activity of the extracts using the agar well diffusion method and preparing plant extract-infused gauze and ointment. Different solvents were used to extract the plant compounds using the Soxhlet apparatus. Antimicrobial activity of the extracts was tested against microorganisms isolated from infected wound samples, cultivated on various media (Nutrient agar, Cetrimide agar, Blood agar, MacConkey agar, and MRSA). Minimum inhibitory concentration was determined using the agar well diffusion method, identifying the antimicrobial efficacy of the plant extracts. The ethanolic extract of Tridax procumbens exhibited the highest antimicrobial activity against Staphylococcus aureus and Escherichia coli, among other test organisms (Streptococcus, Pseudomonas, Proteus sp.,) isolated from the infected wound samples. Phytochemical tests were conducted to identify bioactive compounds responsible for wound healing, which were subsequently isolated using column chromatography and purified via thin layer chromatography. FTIR analysis was performed to determine the functional groups present, and HPLC was conducted for further characterization. The presence of flavonoids, tannins and terpenoids in the plant extracts was identified as the active ingredients responsible for wound healing. Furthermore, a gauze infused with plant extracts was prepared to facilitate wound healing and an ointment was formulated using the ethanolic extract of Tridax procumbens and an ointment base.  
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来源期刊
International Journal of Ayurvedic Medicine
International Journal of Ayurvedic Medicine MEDICINE, RESEARCH & EXPERIMENTAL-
自引率
50.00%
发文量
87
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