乳香对 3-NP 诱发的实验性亨廷顿氏病的神经保护作用

Q2 Pharmacology, Toxicology and Pharmaceutics

Current Bioactive Compounds Pub Date : 2024-01-18 DOI:10.2174/0115734072272233231119161319

Vinay Kumar, Chanchal Sharma, M. Taleuzzaman, Kandasamy Nagarajan, Anzarul Haque, Mamta Bhatia, Sumayya Khan, Mohammad Ayman A. Salkini, pankaj bhatt

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引用次数: 0

摘要

该研究旨在评估血清乳香对 3-NP 诱导的实验性亨廷顿氏病的神经保护作用。本研究以 45 毫克/千克、90 毫克/千克和 180 毫克/千克的口服剂量检测了乳香对 3-硝基丙酸（3-NP）诱导的亨廷顿氏病（HD）的潜在神经保护作用。在这项研究中，3-NP 以 10 毫克/千克的剂量诱导 Wistar 大鼠患上 HD。该研究使用了 56 只 Wistar 大鼠（每组 8 只）进行生化（炎症标志物、乙酰胆碱酯酶活性）和行为（高架加迷宫、Y 型迷宫、开放场、尾悬试验等）评估。此外，还对大脑进行了组织学检查。在大脑中，琥珀酸脱氢酶是一种被 3-NP 不可逆抑制的线粒体酶。给大鼠服用 3-NP 会导致 HD，并使其行为和运动发生改变。使用血清乳香（Boswellia serrata）治疗可显著保护大鼠免受 3-NP 引起的行为和运动障碍的影响，且这种影响与剂量有关。此外，在给大鼠注射 3-NP 后，乳香血清片还能改善其记忆能力并降低炎症生物标志物的水平。研究发现，乙酰基-11-酮基对乳香酸是乳香提取物的主要活性成分。与其他剂量的乳香提取物相比，180 毫克/千克剂量的乳香提取物对 3-NP 诱导的 HD 有更好的保护作用。要将乳香提取物从实验室应用到临床，将其用作HD患者的辅助药物，还需要基于分子靶点进行更详细的研究。

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Neuroprotective Effect of Boswellia serrata against 3-NP Induced Experimental Huntington’s Disease

The study aimed to assess the neuroprotective effect of Boswellia serrata against 3-NP-induced experimental Huntington’s disease. Previous studies have shown Boswellia to have sedative, analgesic, and anti-tumour effects. Boswellia serrata yields four pentacyclic triterpene acids and boswellic acid, a bioactive substance that prevents leukotriene biogenesis. The potential neuroprotective effect of Boswellia serrata against 3-nitro propionic acid (3-NP)-induced Huntington's disease (HD) was examined at oral doses of 45 mg/kg, 90 mg/kg, and 180 mg/kg. In this study, HD was induced by 3-NP at a dose of 10 mg/kg in Wistar rats. The study used 56 Wistar rats (8 per group) for biochemical (inflammatory markers, acetylcholinesterase activity) and behavioural (elevated plus maze, Y-maze, open-field, tail suspension tests, etc.) assessments. Additionally, a histological examination of the brain was carried out. In addition, the analysis of Boswellia serrata extract was performed by different analytical techniques, like UV spectrophotometer, FTIR, and HPLC methods. In the brain, succinate dehydrogenase is a mitochondrial enzyme irreversibly inhibited by 3-NP. Administration of 3-NP resulted in HD with altered behavioural and motor changes in rats. Treatment with Boswellia serrata resulted in remarkable protection of rats against 3-NP-induced behaviour and motor deficits in a dose-dependent manner. Moreover, in rats administered with 3-NP, Boswellia serrata improved memory performance and lowered levels of inflammatory biomarkers. These results have also been supported by histopathological analysis. Acetyl-11-keto-p-boswellic acid was found to be the main active component of Boswellia serrata extract. Boswellia serrata at a dose of 180 mg/kg exhibited better protection compared to the other doses against HD induced by 3-NP. More detailed studies based on molecular targets are needed for the Boswellia serrata to transition from the bench to the bedside for use as an adjuvant in HD patients.

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来源期刊

Current Bioactive Compounds Pharmacology, Toxicology and Pharmaceutics-Pharmacology, Toxicology and Pharmaceutics (all)

CiteScore

1.90

自引率

0.00%

发文量

112

期刊介绍： The journal aims to provide comprehensive review articles on new bioactive compounds with proven activities in various biological screenings and pharmacological models with a special emphasis on stereoeselective synthesis. The aim is to provide a valuable information source of bioactive compounds synthesized or isolated, which can be used for further development of pharmaceuticals by industry and academia. The journal should prove to be essential reading for pharmacologists, natural product chemists and medicinal chemists who wish to be kept informed and up-to-date with the most important developments on new bioactive compounds of natural or synthetic origin, including their stereoeselective synthesis.