CRISPR 介导的 OsNAS2 顺式调控元件启动子编辑可提高水稻的锌吸收/转运能力和植物产量

IF 4.9 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Yvonne Ludwig, C. Dueñas, E. Arcillas, Reena Jesusa Macalalad-Cabral, Ajay Kohli, Russell Reinke, I. Slamet-Loedin
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引用次数: 0

摘要

开发产量更高的营养水稻是缓解发展中国家微量营养素缺乏问题(尤其是涉及锌和铁(Fe)缺乏的人类营养不良问题)和实现更好采用的方法之一。铁和锌等微量营养元素的转运主要通过烟酰胺合成酶(OsNAS)基因家族调控,而产量则是一个涉及多个基因位点的复杂性状。为了提高谷粒和单株的锌积累,我们通过CRISPR(簇状规则间隔短回文重复)-Cas9对OsNAS2启动子进行了基因组编辑,特别是删除了位于-933位的顺式调控元件ARR1AT。结果表明,我们的启动子编辑增加了每株植物的锌浓度。证据还显示,每株主圆锥花序的小穗数增加也会导致单株籽粒增加。这些性状在 "无转基因 "和同源植物后代中得到了遗传。要验证田间条件下的性状表现并阐明小穗增加的原因,还需要进行进一步的调查。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
CRISPR-mediated promoter editing of a cis-regulatory element of OsNAS2 increases Zn uptake/translocation and plant yield in rice
Developing nutritious rice with a higher yield is one approach to alleviating the problem of micronutrient deficiency in developing countries, especially human malnutrition involving zinc and iron (Fe) deficiency, and achieving better adoption. The transport of micronutrients such as Fe and Zn is mainly regulated via the nicotianamine synthase (OsNAS) gene family, whereas yield is a complex trait that involves multiple loci. Genome editing via CRISPR (clustered regularly interspaced short palindromic repeat)-Cas9, focusing on the OsNAS2 promoter, particularly the deletion of the cis-regulatory element ARR1AT at position −933, was conducted for an enhanced accumulation of Zn in the grain and per plant. The results showed that our promoter editing increased Zn concentration per plant. Evidence also showed that an improved spikelet number per main panicle led to increased grain per plant. The traits were inherited in “transgene-free” and homozygous plant progenies. Further investigation needs to be conducted to validate trait performance under field conditions and elucidate the cause of the spikelet increase.
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CiteScore
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