{"title":"石蝇染色体的新发现:I. 西伯利亚 Skwala compacta (McLachlan, 1872)(多翅目,鳞翅目,石蝇科)的核型、C-带和核糖体及端粒 DNA 标记的定位","authors":"A. Bugrov, T. Karamysheva, Olesya Buleu","doi":"10.3897/compcytogen.18.115784","DOIUrl":null,"url":null,"abstract":"This study provides data on chromosome number (2n♂♀=26), sex determination mechanism (XY♂/XX♀), C-banding pattern, distribution of clusters of telomeric TTAGG repeats and 18S ribosomal DNA in the karyotype of the stonefly Skwala compacta (McLachlan, 1872). For the first time in the history of stoneflies cytogenetics, we provide photos of the chromosomes of the Plecoptera insects. The karyotype of males and females of S. compacta consists of 12 pairs of autosomes. Three pairs of large autosomes and four pairs of medium-sized autosomes are subacrocentric. The remaining pairs of autosomes are small, with unclear morphology. Pericentromeric C-bands were revealed in all autosomes. The sex chromosomes are also subacrocentric. The short arms of X and Y chromosomes are entirely heterochromatic and are rich in ribosomal DNA sequences. In the X chromosome this arm is larger than in the Y chromosome. It is likely that this arm associated with the nucleolar organizer (NOR). Telomeric DNA (TTAGG)n repeats were detected in the terminal regions of all chromosomes.","PeriodicalId":50656,"journal":{"name":"Comparative Cytogenetics","volume":null,"pages":null},"PeriodicalIF":1.0000,"publicationDate":"2024-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"New insights into the chromosomes of stoneflies: I. Karyotype, C-banding and localization of ribosomal and telomeric DNA markers in Skwala compacta (McLachlan, 1872) (Polyneoptera, Plecoptera, Perlodidae) from Siberia\",\"authors\":\"A. Bugrov, T. Karamysheva, Olesya Buleu\",\"doi\":\"10.3897/compcytogen.18.115784\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"This study provides data on chromosome number (2n♂♀=26), sex determination mechanism (XY♂/XX♀), C-banding pattern, distribution of clusters of telomeric TTAGG repeats and 18S ribosomal DNA in the karyotype of the stonefly Skwala compacta (McLachlan, 1872). For the first time in the history of stoneflies cytogenetics, we provide photos of the chromosomes of the Plecoptera insects. The karyotype of males and females of S. compacta consists of 12 pairs of autosomes. Three pairs of large autosomes and four pairs of medium-sized autosomes are subacrocentric. The remaining pairs of autosomes are small, with unclear morphology. Pericentromeric C-bands were revealed in all autosomes. The sex chromosomes are also subacrocentric. The short arms of X and Y chromosomes are entirely heterochromatic and are rich in ribosomal DNA sequences. In the X chromosome this arm is larger than in the Y chromosome. It is likely that this arm associated with the nucleolar organizer (NOR). Telomeric DNA (TTAGG)n repeats were detected in the terminal regions of all chromosomes.\",\"PeriodicalId\":50656,\"journal\":{\"name\":\"Comparative Cytogenetics\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.0000,\"publicationDate\":\"2024-01-25\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Comparative Cytogenetics\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.3897/compcytogen.18.115784\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"GENETICS & HEREDITY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Comparative Cytogenetics","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.3897/compcytogen.18.115784","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"GENETICS & HEREDITY","Score":null,"Total":0}
引用次数: 0
摘要
本研究提供了石蝇 Skwala compacta(McLachlan,1872 年)核型中染色体数目(2n♂♀=26)、性别决定机制(XY♂/XX♀)、C 带模式、端粒 TTAGG 重复群分布和 18S 核糖体 DNA 的数据。在石蝇细胞遗传学的历史上,我们首次提供了褶翅目昆虫染色体的照片。紧凑石蝇雄性和雌性的核型由 12 对常染色体组成。其中三对大型常染色体和四对中型常染色体是亚同心的。其余几对常染色体较小,形态不清晰。在所有常染色体上都发现了近中心 C 带。性染色体也是近心形。X 和 Y 染色体的短臂完全是异染色质,富含核糖体 DNA 序列。X 染色体的短臂比 Y 染色体的大。该臂很可能与核极组织者(NOR)有关。在所有染色体的末端区域都检测到了端粒 DNA (TTAGG)n 重复序列。
New insights into the chromosomes of stoneflies: I. Karyotype, C-banding and localization of ribosomal and telomeric DNA markers in Skwala compacta (McLachlan, 1872) (Polyneoptera, Plecoptera, Perlodidae) from Siberia
This study provides data on chromosome number (2n♂♀=26), sex determination mechanism (XY♂/XX♀), C-banding pattern, distribution of clusters of telomeric TTAGG repeats and 18S ribosomal DNA in the karyotype of the stonefly Skwala compacta (McLachlan, 1872). For the first time in the history of stoneflies cytogenetics, we provide photos of the chromosomes of the Plecoptera insects. The karyotype of males and females of S. compacta consists of 12 pairs of autosomes. Three pairs of large autosomes and four pairs of medium-sized autosomes are subacrocentric. The remaining pairs of autosomes are small, with unclear morphology. Pericentromeric C-bands were revealed in all autosomes. The sex chromosomes are also subacrocentric. The short arms of X and Y chromosomes are entirely heterochromatic and are rich in ribosomal DNA sequences. In the X chromosome this arm is larger than in the Y chromosome. It is likely that this arm associated with the nucleolar organizer (NOR). Telomeric DNA (TTAGG)n repeats were detected in the terminal regions of all chromosomes.
期刊介绍:
Comparative Cytogenetics is a peer-reviewed, open-access, rapid online journal launched to accelerate research on all aspects of plant and animal cytogenetics, karyosystematics, and molecular systematics.
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