Jeremiah Leach, Hannah N Suber, Katelyn Conley, Regan Rivera, James Surles, Benjamin Hames, Ronald J Kendall
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Validation of an enzyme immunoassay for the detection of corticosterone metabolites from northern bobwhite (Colinus virginianus) feces
Interest in the effects of stressors on wildlife has grown substantially over the past few decades. As this interest has grown, so has the need for minimally invasive and reliable methods for estimating differences in the levels of stress hormones. An enzyme immunoassay using standardized methods was validated for detecting concentrations of corticosterone (cort) metabolites from northern bobwhite fecal samples. Two physiological challenges and one biological challenge were applied to 18 northern bobwhites (nine males and nine females), and the fecal cort metabolite concentrations were compared to baseline levels. The interactions of sex and treatment, treatment and time and sex and time were all significant. Thus, the methods and tools used here were sensitive enough to detect expected changes to the hypothalamo–pituitary–adrenal axis of northern bobwhite.
期刊介绍:
Conservation Physiology is an online only, fully open access journal published on behalf of the Society for Experimental Biology.
Biodiversity across the globe faces a growing number of threats associated with human activities. Conservation Physiology will publish research on all taxa (microbes, plants and animals) focused on understanding and predicting how organisms, populations, ecosystems and natural resources respond to environmental change and stressors. Physiology is considered in the broadest possible terms to include functional and mechanistic responses at all scales. We also welcome research towards developing and refining strategies to rebuild populations, restore ecosystems, inform conservation policy, and manage living resources. We define conservation physiology broadly and encourage potential authors to contact the editorial team if they have any questions regarding the remit of the journal.