验证用于检测北部山齿鹑(Colinus virginianus)粪便中皮质酮代谢物的酶免疫分析法

IF 2.6 3区 环境科学与生态学 Q2 BIODIVERSITY CONSERVATION
Jeremiah Leach, Hannah N Suber, Katelyn Conley, Regan Rivera, James Surles, Benjamin Hames, Ronald J Kendall
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引用次数: 0

摘要

在过去的几十年里,人们对压力因素对野生动物的影响的兴趣大大增加。随着这种兴趣的增长,人们也需要采用微创、可靠的方法来估计应激激素水平的差异。采用标准化方法对酶免疫测定进行了验证,以检测北部山齿白鸟粪便样本中皮质酮(cort)代谢物的浓度。对 18 只北方白冠鹿(9 雄 9 雌)进行了两次生理挑战和一次生物挑战,并将粪便中皮质代谢物的浓度与基线水平进行了比较。性别与处理、处理与时间以及性别与时间的交互作用均有显著性。因此,本文使用的方法和工具足够灵敏,可以检测到北部山齿鹬下丘脑-垂体-肾上腺轴的预期变化。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Validation of an enzyme immunoassay for the detection of corticosterone metabolites from northern bobwhite (Colinus virginianus) feces
Interest in the effects of stressors on wildlife has grown substantially over the past few decades. As this interest has grown, so has the need for minimally invasive and reliable methods for estimating differences in the levels of stress hormones. An enzyme immunoassay using standardized methods was validated for detecting concentrations of corticosterone (cort) metabolites from northern bobwhite fecal samples. Two physiological challenges and one biological challenge were applied to 18 northern bobwhites (nine males and nine females), and the fecal cort metabolite concentrations were compared to baseline levels. The interactions of sex and treatment, treatment and time and sex and time were all significant. Thus, the methods and tools used here were sensitive enough to detect expected changes to the hypothalamo–pituitary–adrenal axis of northern bobwhite.
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来源期刊
Conservation Physiology
Conservation Physiology Environmental Science-Management, Monitoring, Policy and Law
CiteScore
5.10
自引率
3.70%
发文量
71
审稿时长
11 weeks
期刊介绍: Conservation Physiology is an online only, fully open access journal published on behalf of the Society for Experimental Biology. Biodiversity across the globe faces a growing number of threats associated with human activities. Conservation Physiology will publish research on all taxa (microbes, plants and animals) focused on understanding and predicting how organisms, populations, ecosystems and natural resources respond to environmental change and stressors. Physiology is considered in the broadest possible terms to include functional and mechanistic responses at all scales. We also welcome research towards developing and refining strategies to rebuild populations, restore ecosystems, inform conservation policy, and manage living resources. We define conservation physiology broadly and encourage potential authors to contact the editorial team if they have any questions regarding the remit of the journal.
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