利用带二极管阵列检测器的高效液相色谱法分析不同加工方法对拉尼氏输卵管中主要有效成分的影响

IF 2.8 4区化学 Q2 CHEMISTRY, MULTIDISCIPLINARY

Journal of Chemistry Pub Date : 2024-01-23 DOI:10.1155/2024/3524367

Yang Xu, Xianwen Yue, Jihong Chi, Huailei Yang, Ying Wang, Yongsheng Wang, Peng Yu, Huiwei Bao

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引用次数: 0

摘要

乌药是中国 "长白山瑰宝 "之一。临床用于治疗病后体虚、神疲乏力、咳嗽、咯血等症。本研究建立了一种简便灵敏的高效液相色谱-二极管阵列检测器（HPLC-DAD）方法，用于同时测定 OR 及其不同加工品中的五种主要有效成分（1-甲基海因、雌二醇、胆固醇、7-酮基胆固醇和 4-胆甾烯-3-酮）。结果表明，这五种成分在各自的浓度范围内呈良好的线性关系，测定系数≥0.9996。平均回收率为 97.67% 至 100.06%，RSD 为 1.45% 至 1.99%。该方法简便、准确、稳定，为 OR 及其不同加工产品的质量控制和评价提供了一种有效的分析方法。

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Analysis of the Influence of Different Processing Methods on the Main Effective Components in Oviductus Ranae by High-Performance Liquid Chromatography with Diode Array Detector

Oviductus Ranae (OR) is one of the “treasures of Changbai Mountain” in China. It is used clinically for the treatment of infirmity after illness, fatigued spirit, cough, hemoptysis, and so forth. In this study, a simple and sensitive high-performance liquid chromatography-diode array detector (HPLC-DAD) method was developed for the simultaneous determination of five main effective components (1-methyl hydantoin, estradiol, cholesterol, 7-keto-cholesterol, and 4-cholesten-3-one) in OR and its different processed products. The results indicated that these five components showed good linear relationships within their own concentration ranges along with coefficients of determination ≥0.9996. Average recoveries ranged from 97.67% to 100.06%, with RSDs of 1.45%–1.99%. The proposed method was found to be simple, accurate, and stable, which provided an effective analytical method for quality control and evaluation of OR and its different processed products.

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来源期刊

Journal of Chemistry CHEMISTRY, MULTIDISCIPLINARY-

CiteScore

5.90

自引率

3.30%

发文量

345

审稿时长

16 weeks

期刊介绍： Journal of Chemistry is a peer-reviewed, Open Access journal that publishes original research articles as well as review articles in all areas of chemistry.