Taiki Mori , Cong Wang , Senhao Wang , Wei Zhang , Jiangming Mo
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引用次数: 0
摘要
β-葡萄糖苷酶(BG)与磷单酯酶(PME)活性之比(BG:PME)通常被用来预测微生物缺磷(P)的强度,BG:PME越低表明缺磷越强(酶的化学计量理论)。在这里,我们证明了连续 10 年以 NaH2PO4 溶液形式施用高达 150 kg P ha-1 yr-1 的磷肥并不会使 BG:PME 提高到其他陆地生态系统的水平。在施过钾肥的地块中,原始林、次生林和人工林的 BG:PME 分别为 0.094、0.067 和 0.089,远低于全球平均水平(0.62 ± 0.04),尽管布雷提取的钾含量大幅提高(超过 600 倍)。因此,本研究的结果表明,BG:PME 高估了我国富含 P 的森林中 P 的缺乏程度,这意味着酶的化学计量理论可能并不普遍适用。
Contradiction with enzymatic stoichiometry theory: Persistent low ratios of β-glucosidase to phosphomonoesterase following 10-year continuous phosphorus fertilization in three subtropical forests
The ratio of β-glucosidase (BG) to phosphomonoesterase (PME) activity (BG:PME) is often used to predict the intensity of microbial phosphorus (P) shortage, with lower BG:PME indicating stronger P shortage (enzymatic stoichiometry theory). Here, we demonstrated that 10-year continuous P fertilization as high as 150 kg P ha−1 yr−1 in the form of NaH2PO4 solution did not elevate the BG:PME up to the level of other terrestrial ecosystems. The BG:PME of primary, secondary, and planted forests were 0.094, 0.067, and 0.089, respectively in P-fertilized plots, which were much lower than global average (0.62 ± 0.04), despite the fact that Bray-extracted P contents were substantially elevated (more than 600 times). Thus, the findings of the current study suggest that BG:PME overestimates P shortage in our P-enriched forests, implying that the enzymatic stoichiometry theory may not be universally applicable.
期刊介绍:
Pedobiologia publishes peer reviewed articles describing original work in the field of soil ecology, which includes the study of soil organisms and their interactions with factors in their biotic and abiotic environments.
Analysis of biological structures, interactions, functions, and processes in soil is fundamental for understanding the dynamical nature of terrestrial ecosystems, a prerequisite for appropriate soil management. The scope of this journal consists of fundamental and applied aspects of soil ecology; key focal points include interactions among organisms in soil, organismal controls on soil processes, causes and consequences of soil biodiversity, and aboveground-belowground interactions.
We publish:
original research that tests clearly defined hypotheses addressing topics of current interest in soil ecology (including studies demonstrating nonsignificant effects);
descriptions of novel methodological approaches, or evaluations of current approaches, that address a clear need in soil ecology research;
innovative syntheses of the soil ecology literature, including metaanalyses, topical in depth reviews and short opinion/perspective pieces, and descriptions of original conceptual frameworks; and
short notes reporting novel observations of ecological significance.